From 16941a966808a5102a775ce8e2c00f0c92050f22 Mon Sep 17 00:00:00 2001 From: "marcin p. joachimiak" <4625870+realmarcin@users.noreply.github.com> Date: Tue, 6 Oct 2026 13:28:01 -0700 Subject: [PATCH] Qualify KZ and kombucha causal claims and record research gaps --- ...bacter_KZ_Phenanthrene_Cadmium_SynCom.html | 76 +- ...li_Coinducible_Biosynthesis_Coculture.html | 17 +- ...ucha_KMC_IMBG1_Fermentation_Community.html | 179 +++- .../2026-10-06T201911Z-codex-4edaec.yaml | 25 + .../2026-10-06T201911Z-codex-e2d63d.yaml | 24 + .../2026-10-06T201911Z-codex-36fc6c.yaml | 25 + ...bacter_KZ_Phenanthrene_Cadmium_SynCom.yaml | 89 +- ...li_Coinducible_Biosynthesis_Coculture.yaml | 34 +- ...ucha_KMC_IMBG1_Fermentation_Community.yaml | 139 ++- reports/causal_graph_review/README.md | 21 + .../adversarial-review-20261006-batch60.yaml | 179 ++++ .../20261006-kz-kmc-komagataella-batch60.yaml | 206 +++++ reports/causal_graph_review/inventory.tsv | 6 +- reports/causal_graph_review/summary.json | 8 +- .../validation-20261006-batch60.json | 814 ++++++++++++++++++ ...culture-edison-literature-causal-meta.yaml | 128 +++ tests/test_causal_review_batch60.py | 143 +++ tests/test_participating_taxa.py | 2 + 18 files changed, 1971 insertions(+), 144 deletions(-) create mode 100644 history/records/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom/2026-10-06T201911Z-codex-4edaec.yaml create mode 100644 history/records/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture/2026-10-06T201911Z-codex-e2d63d.yaml create mode 100644 history/records/Kombucha_KMC_IMBG1_Fermentation_Community/2026-10-06T201911Z-codex-36fc6c.yaml create mode 100644 reports/causal_graph_review/adversarial-review-20261006-batch60.yaml create mode 100644 reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml create mode 100644 reports/causal_graph_review/validation-20261006-batch60.json create mode 100644 research/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-edison-literature-causal-meta.yaml create mode 100644 tests/test_causal_review_batch60.py diff --git a/docs/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.html b/docs/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.html index 258d2afa9..43700395b 100644 --- a/docs/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.html +++ b/docs/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.html @@ -602,7 +602,7 @@

Ecological Interactions

Ecological interaction network for Klebsiella-Arthrobacter KZ Phenanthrene-Cadmium SynCom - Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (niche partitioning). + Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction.
@@ -610,8 +610,8 @@

Ecological Interactions

Niche partitioning + aria-hidden="true" data-symbol="symbolTriangle2" + data-color="#929caa"> Other
@@ -619,17 +619,20 @@

Ecological Interactions

-

SZ-3 to CW-D3T phenanthrene-mineralization division of labor

- NICHE_PARTITIONING +

Assay-Inferred Phenanthrene Catabolic Division Of Labor

-

Arthrobacter sp. SZ-3 supplied stronger upstream catalytic activity, measured as 50% higher 2H1N conversion, whereas Klebsiella sp. CW-D3T dominated downstream mineralization with more than 80% contribution in the biomass-normalized enzyme activity assays.

+

Under 25 mg/L cadmium stress, the KZ pair showed better phenanthrene degradation and mineralization than the monocultures. Biomass-normalized enzyme assays assign stronger upstream 2H1N conversion to Arthrobacter sp. SZ-3 and a dominant downstream mineralization contribution to Klebsiella sp. CW-D3T. PARTIAL - these complementary activities support a division-of-labor model, not a resolved donor-to-recipient transfer or necessary mediation. The linked corrigendum and complete assay Methods remain unverified.

-

Source Taxon: Arthrobacter sp. SZ-3

+

Participating Taxa: -

Target Taxon: Klebsiella sp. CW-D3T

+ Klebsiella sp. CW-D3T, + + Arthrobacter sp. SZ-3 + +

@@ -656,13 +659,26 @@

Evidence

PMID:41365368 - - SUPPORT (IN_VITRO) + - PARTIAL (IN_VITRO)
"Through biomass-normalized enzyme activity assays, we uncovered a structured division of labor: SZ-3 exhibited superior upstream catalytic activity (50 % higher 2H1N conversion), while CW-D3T dominated downstream mineralization (>80 % contribution)."
+
  • +
    + + PMID:41365368 + + - SUPPORT (IN_VITRO) +
    + +
    "which synergistically enhanced phenanthrene (PHE) degradation and mineralization under cadmium stress (25 mg/L Cd2+), outperforming monocultures by 1.2-1.9-fold."
    + +
  • + @@ -673,7 +689,7 @@

    Evidence

    -

    Knowledge gaps & discussions (1)

    +

    Knowledge gaps & discussions (2)

    @@ -688,6 +704,21 @@

    +

    + +
    +

    + KNOWLEDGE_GAP + OPEN + What does the corrigendum change, and which transfers mediate KZ performance? +

    +

    The complete abstract and public publisher preview support positive KZ outcomes and assay-inferred upstream/downstream roles. Full enzyme Methods, supplements and tracer or selective perturbation evidence were not read. PMID:41456976 / doi:10.1016/j.biortech.2025.133853 is a linked corrigendum; the official publisher API supplied metadata only, not its body. Do not assume the correction is biologically immaterial or infer expression from metagenomic gene content. #1541 tracks unresolved verification. Keep this record needs_research after the bounded #1540 repair. Existing medium/inoculation questions remain open; routine LB/MSM mentioned in the preview is not a verified co-contamination assay recipe.

    + +

    Attaches to: ecological_interactions#Assay-Inferred Phenanthrene Catabolic Division Of Labor

    + + + +
    @@ -934,7 +965,7 @@

    Evidence

    (function() { var intId = "interaction:0"; - var intType = "NICHE_PARTITIONING"; + var intType = "UNKNOWN"; var metabolites = []; @@ -943,11 +974,11 @@

    Evidence

    var processes = []; - var evidenceCount = 1; + var evidenceCount = 2; nodes.push({ id: intId, - label: "SZ-3 to CW-D3T phenanthrene-mineralization division of labor", + label: "Assay-Inferred Phenanthrene Catabolic Division Of Labor", type: "interaction", interactionType: intType, metabolites: metabolites, @@ -956,17 +987,20 @@

    Evidence

    }); - var srcKey = "Arthrobacter sp. SZ-3"; - if (taxonNodeIds[srcKey]) { - links.push({ source: taxonNodeIds[srcKey], target: intId }); - } + var participantKeys = [ - var tgtKey = "Klebsiella sp. CW-D3T"; - if (taxonNodeIds[tgtKey]) { - links.push({ source: intId, target: taxonNodeIds[tgtKey] }); - } + "Klebsiella sp. CW-D3T", + + "Arthrobacter sp. SZ-3" + + ]; + participantKeys.forEach(function(key) { + if (taxonNodeIds[key]) { + links.push({ source: taxonNodeIds[key], target: intId }); + } + }); })(); diff --git a/docs/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.html b/docs/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.html index 28eefd86c..43fdcbfde 100644 --- a/docs/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.html +++ b/docs/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.html @@ -549,7 +549,7 @@

    Ecological Interactions

    -

    Knowledge gaps & discussions (1)

    +

    Knowledge gaps & discussions (2)

    @@ -564,6 +564,21 @@

    +

    + +
    +

    + KNOWLEDGE_GAP + OPEN + Which strain-resolved relays and induction responses support a causal biosynthesis graph? +

    +

    The abstract and public publisher Introduction/section previews report compatible co-growth, common-signal induction and relayed simvastatin/naringenin production. A design without engineered population-balancing interactions does not prove biological neutralism; sharing an induction signal is not metabolite cross-feeding. Monacolin J acid and an acyl donor are discussed, but exact chassis/construct assignments, transfer controls and full Methods remain unresolved. #1542 tracks the empty graph as needs_research, not absent biology. A reviewed one-community LITERATURE dry run contains 7,457 characters; zero provider submissions or credits. Specifically authorized research and primary verification are needed before adding new nodes/arrows. Preserve the existing intermediate-assignment discussion and canonical species IDs.

    + +

    Attaches to: ecological_interactions, engineering_design

    + + + +
    diff --git a/docs/communities/Kombucha_KMC_IMBG1_Fermentation_Community.html b/docs/communities/Kombucha_KMC_IMBG1_Fermentation_Community.html index 04e3255f8..f58c9dc7b 100644 --- a/docs/communities/Kombucha_KMC_IMBG1_Fermentation_Community.html +++ b/docs/communities/Kombucha_KMC_IMBG1_Fermentation_Community.html @@ -643,7 +643,7 @@

    Ecological Interactions

    Ecological interaction network for Kombucha KMC-IMBG1 Fermentation Community - Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction (mutualism, niche partitioning, colonization facilitation). + Bipartite graph where circle nodes represent taxa and each ecological interaction is drawn as a distinct non-circular symbol, with colour repeating the same distinction.
    @@ -651,18 +651,8 @@

    Ecological Interactions

    Mutualism -
    -
    - Niche partitioning -
    -
    - Colonization facilitation + aria-hidden="true" data-symbol="symbolTriangle2" + data-color="#929caa"> Other
    @@ -670,14 +660,25 @@

    Ecological Interactions

    -

    Yeast-Acetobacter Mutualistic Fermentation

    - MUTUALISM +

    Proposed Yeast-Acetobacter Metabolic Cooperation

    -

    The kombucha microbial community is described as a bacteria-yeast mutualistic metabolic system, with yeasts and cellulose-forming acetobacteria maintained together in a surface pellicle.

    +

    HYPOTHESIZED - the source describes bacteria-yeast metabolic cooperation as the general KMC model. KMC-IMBG1 cultures contain acetobacteria and yeasts, but this composition/microscopy study does not selectively demonstrate exchanged metabolites or reciprocal growth benefits. The four named core genera represent a larger, condition-dependent assemblage, not an exhaustive identical membership in every culture.

    +

    Participating Taxa: + + Komagataeibacter, + + Gluconobacter, + + Pichia, + + Brettanomyces + +

    + @@ -712,7 +713,7 @@

    Evidence

    PMID:26061774 - - SUPPORT (IN_VITRO) + - PARTIAL (IN_VITRO)
    "mutualistic metabolic cooperation of pro- and eukaryotic microorganisms"
    @@ -726,14 +727,23 @@

    Evidence

    Cellulose Pellicle Community Structuring

    - COLONIZATION_FACILITATION
    -

    Cellulose-producing acetobacteria create a surface pellicle that encloses the mixed yeast and bacterial community, physically structuring biochemical interactions.

    +

    Cellulose-producing bacteria form the surface pellicle, and microscopy reports mixed bacterial and yeast morphotypes associated with its cellulose web. This preserves physical community structuring. PARTIAL - the proposed facilitation of biochemical cooperation is distinct from observed association and was not selectively tested by disrupting the pellicle.

    + + -

    Source Taxon: Komagataeibacter

    +

    Participating Taxa: + Komagataeibacter, + Gluconobacter, + + Pichia, + + Brettanomyces + +

    @@ -756,8 +766,8 @@

    Cellulose Pellicle Community Structuring

    Downstream Effects:
    - → Yeast-Acetobacter Mutualistic Fermentation -

    The cellulose pellicle encloses the mixed yeast-acetobacter culture at the liquid surface, facilitating the close biochemical interplay that maintains kombucha fermentation.

    + → Proposed Yeast-Acetobacter Metabolic Cooperation +

    HYPOTHESIZED - pellicle enclosure could facilitate bacteria-yeast biochemical cooperation through spatial association, as explicitly proposed in the source Introduction. Microscopy supports association, not a selective test that pellicle formation mediates reciprocal benefit.

    @@ -780,19 +790,43 @@

    Evidence

    +
  • +
    + + PMID:26061774 + + - PARTIAL (IN_VITRO) +
    + +
    "Close biochemical interplay between yeasts and bacteria was facilitated by enclosing the mixed community within cellulose-based pellicles"
    + +
  • +
    -

    Growth-Condition Dependent Accessory Recruitment

    - NICHE_PARTITIONING +

    Growth-Condition Dependent Community Composition

    -

    KMC-IMBG1 maintained a stable acetobacterial core but shifted accessory bacteria and yeast composition across sterile sweetened tea, nonsterile sweetened tea, honey-supplemented tea, and cabbage-brine hybrid microenvironments.

    +

    Culture and 16S/ITS profiles change across sterile or nonsterile sweetened tea, honey tea and the cabbage-brine hybrid, with relatively stable acetobacterial membership and more variable yeast composition. Lactobacilli likely introduced with cabbage brine persisted through passages. PARTIAL - active recruitment and added fermentative capacity remain proposed; relative sequence profiles do not establish absolute growth, niche partitioning or reciprocal benefit.

    + +

    Participating Taxa: + + Komagataeibacter, + + Gluconobacter, + + Pichia, + + Brettanomyces + +

    @@ -811,16 +845,6 @@

    Growth-Condition Dependent Accessory Recruitment

    -
    - Downstream Effects: - -
    - → Yeast-Acetobacter Mutualistic Fermentation -

    Growth-condition shifts can recruit accessory bacteria such as lactobacilli that may contribute to the broader KMC fermentative capacity.

    -
    - -
    -

    Evidence

    @@ -896,6 +920,25 @@

    Associated Datasets

    +
    +

    Knowledge gaps & discussions (1)

    + +
    +

    + KNOWLEDGE_GAP + OPEN + Which exchanges and benefits are mediated by the KMC pellicle or accessory members? +

    +

    Retain the source-explicit pellicle-to-cooperation model as HYPOTHESIZED, with physical association distinct from selective mediation. Remove the accessory-composition-to-mutualism arrow: possible fermentation capacity does not establish reciprocal benefit among the canonical core genera. Positive pellicle formation, composition changes and persistence of probable cabbage-derived lactobacilli remain represented. Read the full primary main text and main/supplement captions, not original figure pixels, Table 1 numerical cells, supplementary PDF or raw sequences. Strain-resolved transfer and selective pellicle/accessory perturbations remain open questions. Canonical membership is preserved as a partial core representation; separate non-graph cultivation/ingredient claims remain unresolved under #1543.

    + +

    Attaches to: ecological_interactions#Proposed Yeast-Acetobacter Metabolic Cooperation, ecological_interactions#Cellulose Pellicle Community Structuring, ecological_interactions#Growth-Condition Dependent Community Composition

    + + + + +
    + +
    @@ -1175,7 +1218,7 @@

    Evidence

    (function() { var intId = "interaction:0"; - var intType = "MUTUALISM"; + var intType = "UNKNOWN"; var metabolites = []; var processes = []; @@ -1190,7 +1233,7 @@

    Evidence

    nodes.push({ id: intId, - label: "Yeast-Acetobacter Mutualistic Fermentation", + label: "Proposed Yeast-Acetobacter Metabolic Cooperation", type: "interaction", interactionType: intType, metabolites: metabolites, @@ -1201,11 +1244,28 @@

    Evidence

    + var participantKeys = [ + + "Komagataeibacter", + + "Gluconobacter", + + "Pichia", + + "Brettanomyces" + + ]; + participantKeys.forEach(function(key) { + if (taxonNodeIds[key]) { + links.push({ source: taxonNodeIds[key], target: intId }); + } + }); + })(); (function() { var intId = "interaction:1"; - var intType = "COLONIZATION_FACILITATION"; + var intType = "UNKNOWN"; var metabolites = []; var processes = []; @@ -1214,7 +1274,7 @@

    Evidence

    processes.push("biofilm formation"); - var evidenceCount = 1; + var evidenceCount = 2; nodes.push({ id: intId, @@ -1227,18 +1287,30 @@

    Evidence

    }); - var srcKey = "Komagataeibacter"; - if (taxonNodeIds[srcKey]) { - links.push({ source: taxonNodeIds[srcKey], target: intId }); - } + var participantKeys = [ + + "Komagataeibacter", + + "Gluconobacter", + + "Pichia", + + "Brettanomyces" + + ]; + participantKeys.forEach(function(key) { + if (taxonNodeIds[key]) { + links.push({ source: taxonNodeIds[key], target: intId }); + } + }); })(); (function() { var intId = "interaction:2"; - var intType = "NICHE_PARTITIONING"; + var intType = "UNKNOWN"; var metabolites = []; var processes = []; @@ -1251,7 +1323,7 @@

    Evidence

    nodes.push({ id: intId, - label: "Growth-Condition Dependent Accessory Recruitment", + label: "Growth-Condition Dependent Community Composition", type: "interaction", interactionType: intType, metabolites: metabolites, @@ -1262,6 +1334,23 @@

    Evidence

    + var participantKeys = [ + + "Komagataeibacter", + + "Gluconobacter", + + "Pichia", + + "Brettanomyces" + + ]; + participantKeys.forEach(function(key) { + if (taxonNodeIds[key]) { + links.push({ source: taxonNodeIds[key], target: intId }); + } + }); + })(); diff --git a/history/records/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom/2026-10-06T201911Z-codex-4edaec.yaml b/history/records/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom/2026-10-06T201911Z-codex-4edaec.yaml new file mode 100644 index 000000000..59e960736 --- /dev/null +++ b/history/records/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom/2026-10-06T201911Z-codex-4edaec.yaml @@ -0,0 +1,25 @@ +history_version: 1 +target: + kind: record + path: kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml + slug: Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom +session: + id: 2026-10-06T201911Z-codex-4edaec + timestamp: '2026-10-06T20:19:11Z' + actors: + - type: ai_agent + name: codex + model: unspecified + agent_tool: codex +links: + issues: + - https://github.com/CultureBotAI/CommunityMech/issues/1540 +events: +- type: EDIT + outcome: changed + sections: + - interactions + - discussions + summary: Bound KZ catabolic roles to the reported assays and flag unresolved corrigendum + verification. + details: Source-based self-adversarial review. reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml diff --git a/history/records/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture/2026-10-06T201911Z-codex-e2d63d.yaml b/history/records/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture/2026-10-06T201911Z-codex-e2d63d.yaml new file mode 100644 index 000000000..85e751e0b --- /dev/null +++ b/history/records/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture/2026-10-06T201911Z-codex-e2d63d.yaml @@ -0,0 +1,24 @@ +history_version: 1 +target: + kind: record + path: kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml + slug: Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture +session: + id: 2026-10-06T201911Z-codex-e2d63d + timestamp: '2026-10-06T20:19:11Z' + actors: + - type: ai_agent + name: codex + model: unspecified + agent_tool: codex +links: + issues: + - https://github.com/CultureBotAI/CommunityMech/issues/1542 +events: +- type: EDIT + outcome: changed + sections: + - interactions + - discussions + summary: Expose the unresolved empty Komagataella-E. coli causal biosynthesis graph. + details: Source-based self-adversarial review. reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml diff --git a/history/records/Kombucha_KMC_IMBG1_Fermentation_Community/2026-10-06T201911Z-codex-36fc6c.yaml b/history/records/Kombucha_KMC_IMBG1_Fermentation_Community/2026-10-06T201911Z-codex-36fc6c.yaml new file mode 100644 index 000000000..e593ad559 --- /dev/null +++ b/history/records/Kombucha_KMC_IMBG1_Fermentation_Community/2026-10-06T201911Z-codex-36fc6c.yaml @@ -0,0 +1,25 @@ +history_version: 1 +target: + kind: record + path: kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml + slug: Kombucha_KMC_IMBG1_Fermentation_Community +session: + id: 2026-10-06T201911Z-codex-36fc6c + timestamp: '2026-10-06T20:19:11Z' + actors: + - type: ai_agent + name: codex + model: unspecified + agent_tool: codex +links: + issues: + - https://github.com/CultureBotAI/CommunityMech/issues/1539 +events: +- type: EDIT + outcome: changed + sections: + - interactions + - discussions + summary: Qualify KMC pellicle cooperation and remove the unsupported recruitment-to-mutualism + arrow. + details: Source-based self-adversarial review. reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml diff --git a/kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml b/kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml index 12c70ddff..abcf4a163 100644 --- a/kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml +++ b/kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml @@ -150,41 +150,43 @@ taxonomy: Supports the metagenome-inferred cadmium-stress response assigned to SZ-3. ecological_interactions: -- name: SZ-3 to CW-D3T phenanthrene-mineralization division of labor - description: >- - Arthrobacter sp. SZ-3 supplied stronger upstream catalytic activity, - measured as 50% higher 2H1N conversion, whereas Klebsiella sp. CW-D3T - dominated downstream mineralization with more than 80% contribution in the - biomass-normalized enzyme activity assays. - interaction_type: NICHE_PARTITIONING - scope: PAIRWISE - source_taxon: - preferred_term: Arthrobacter sp. SZ-3 - term: - id: NCBITaxon:1663 - label: Arthrobacter - target_taxon: - preferred_term: Klebsiella sp. CW-D3T - term: - id: NCBITaxon:570 - label: Klebsiella +- name: Assay-Inferred Phenanthrene Catabolic Division Of Labor + description: Under 25 mg/L cadmium stress, the KZ pair showed better phenanthrene degradation and mineralization + than the monocultures. Biomass-normalized enzyme assays assign stronger upstream 2H1N conversion to Arthrobacter + sp. SZ-3 and a dominant downstream mineralization contribution to Klebsiella sp. CW-D3T. PARTIAL - these complementary + activities support a division-of-labor model, not a resolved donor-to-recipient transfer or necessary mediation. + The linked corrigendum and complete assay Methods remain unverified. + scope: COMMUNITY_LEVEL metabolites: - preferred_term: phenanthrene term: id: CHEBI:28851 label: phenanthrene evidence: + - reference: PMID:41365368 + supports: PARTIAL + evidence_source: IN_VITRO + snippet: 'Through biomass-normalized enzyme activity assays, we uncovered a structured division of labor: SZ-3 + exhibited superior upstream catalytic activity (50 % higher 2H1N conversion), while CW-D3T dominated downstream + mineralization (>80 % contribution).' + explanation: Supports complementary assay-inferred roles; does not establish a directed interstrain transfer. + Complete Methods and the linked corrigendum remain unread. - reference: PMID:41365368 supports: SUPPORT evidence_source: IN_VITRO - snippet: >- - Through biomass-normalized enzyme activity assays, we uncovered a - structured division of labor: SZ-3 exhibited superior upstream catalytic - activity (50 % higher 2H1N conversion), while CW-D3T dominated downstream - mineralization (>80 % contribution). - explanation: >- - Supports complementary upstream and downstream phenanthrene-catabolism - roles for the two KZ members. + snippet: which synergistically enhanced phenanthrene (PHE) degradation and mineralization under cadmium stress + (25 mg/L Cd2+), outperforming monocultures by 1.2-1.9-fold. + explanation: Preserves the reported positive consortium-versus-monoculture outcome without assigning its necessary + mediator. + participating_taxa: + - preferred_term: Klebsiella sp. CW-D3T + term: + id: NCBITaxon:570 + label: Klebsiella + - preferred_term: Arthrobacter sp. SZ-3 + term: + id: NCBITaxon:1663 + label: Arthrobacter environmental_factors: - name: Cadmium co-contamination stress value: '25' @@ -237,23 +239,42 @@ external_resources: explanation: Lists the primary publication for the KZ consortium. discussions: - discussion_id: kz_cultivation_and_inoculation_details - prompt: >- - Which growth medium, inoculation ratio, and initial phenanthrene - concentration were used to assemble and assay the KZ consortium? + prompt: Which growth medium, inoculation ratio, and initial phenanthrene concentration were used to assemble and + assay the KZ consortium? kind: KNOWLEDGE_GAP status: OPEN attaches_to: - engineering_design#assembly_strategy - rationale: >- - The PubMed abstract identifies the two KZ members, the 25 mg/L cadmium - stressor, and the phenanthrene-mineralization phenotype, but it does not - expose the medium recipe, inoculum composition, or phenanthrene dose needed - for a fully reproducible cultivation record. + rationale: The PubMed abstract identifies the two KZ members, the 25 mg/L cadmium stressor, and the phenanthrene-mineralization + phenotype, but it does not expose the medium recipe, inoculum composition, or phenanthrene dose needed for a + fully reproducible cultivation record. posed_by: codex posed_date: '2026-09-11' +- discussion_id: kz_correction_and_transfer_verification + prompt: What does the corrigendum change, and which transfers mediate KZ performance? + kind: KNOWLEDGE_GAP + status: OPEN + attaches_to: + - ecological_interactions#Assay-Inferred Phenanthrene Catabolic Division Of Labor + rationale: 'The complete abstract and public publisher preview support positive KZ outcomes and assay-inferred + upstream/downstream roles. Full enzyme Methods, supplements and tracer or selective perturbation evidence were + not read. PMID:41456976 / doi:10.1016/j.biortech.2025.133853 is a linked corrigendum; the official publisher + API supplied metadata only, not its body. Do not assume the correction is biologically immaterial or infer expression + from metagenomic gene content. #1541 tracks unresolved verification. Keep this record needs_research after the + bounded #1540 repair. Existing medium/inoculation questions remain open; routine LB/MSM mentioned in the preview + is not a verified co-contamination assay recipe.' + posed_by: codex + posed_date: '2026-10-06' metals_present: [] rare_earth_elements_present: [] metal_relevance: SIGNIFICANT metal_notes: >- Cadmium is a reported 25 mg/L co-stressor in the KZ phenanthrene assay; the current MetalElementEnum does not include cadmium as an enum value. +curation_history: +- timestamp: '2026-10-06T20:19:10Z' + curator: codex + action: REVIEW_CAUSAL_GRAPH + changes: 'Bound KZ catabolic roles to the reported assays and flag unresolved corrigendum verification. Issue + #1540; reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml' + llm_assisted: true diff --git a/kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml b/kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml index a1b81bd0d..8cf2780c9 100644 --- a/kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml +++ b/kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml @@ -164,23 +164,43 @@ external_resources: co-inducible biosynthesis coculture. discussions: - discussion_id: kphaffii_ecoli_relayed_intermediate - prompt: >- - Which biosynthetic intermediate or intermediates pass between K. phaffii - and E. coli during simvastatin and naringenin production? + prompt: Which biosynthetic intermediate or intermediates pass between K. phaffii and E. coli during simvastatin + and naringenin production? kind: KNOWLEDGE_GAP status: OPEN attaches_to: - engineering_design#assembly_strategy - rationale: >- - The abstract reports relayed synthesis and single-signal co-induction in - coculture, but the accessible cache does not identify the exchanged - metabolite or assign each reporter pathway segment to K. phaffii versus + rationale: The abstract reports relayed synthesis and single-signal co-induction in coculture, but the accessible + cache does not identify the exchanged metabolite or assign each reporter pathway segment to K. phaffii versus E. coli. posed_by: codex posed_date: '2026-09-11' +- discussion_id: komagataella_ecoli_causal_graph_extension + prompt: Which strain-resolved relays and induction responses support a causal biosynthesis graph? + kind: KNOWLEDGE_GAP + status: OPEN + attaches_to: + - ecological_interactions + - engineering_design + rationale: 'The abstract and public publisher Introduction/section previews report compatible co-growth, common-signal + induction and relayed simvastatin/naringenin production. A design without engineered population-balancing interactions + does not prove biological neutralism; sharing an induction signal is not metabolite cross-feeding. Monacolin + J acid and an acyl donor are discussed, but exact chassis/construct assignments, transfer controls and full + Methods remain unresolved. #1542 tracks the empty graph as needs_research, not absent biology. A reviewed one-community + LITERATURE dry run contains 7,457 characters; zero provider submissions or credits. Specifically authorized + research and primary verification are needed before adding new nodes/arrows. Preserve the existing intermediate-assignment + discussion and canonical species IDs.' + posed_by: codex + posed_date: '2026-10-06' metals_present: [] rare_earth_elements_present: [] metal_relevance: NOT_APPLICABLE metal_notes: >- No metal or rare-earth processing role is reported for the K. phaffii-E. coli co-inducible biosynthesis coculture. +curation_history: +- timestamp: '2026-10-06T20:19:10Z' + curator: codex + action: REVIEW_CAUSAL_GRAPH + changes: 'Expose the unresolved empty Komagataella-E. coli causal biosynthesis graph. Issue #1542; reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml' + llm_assisted: true diff --git a/kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml b/kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml index 412e6ec36..c9587aaa0 100644 --- a/kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml +++ b/kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml @@ -104,10 +104,11 @@ taxonomy: snippet: Brettanomyces/Dekkera and Pichia (including former Issatchenkia) were dominant explanation: Supports Brettanomyces/Dekkera as a dominant yeast lineage in KMC-IMBG1. ecological_interactions: -- name: Yeast-Acetobacter Mutualistic Fermentation - description: The kombucha microbial community is described as a bacteria-yeast mutualistic metabolic - system, with yeasts and cellulose-forming acetobacteria maintained together in a surface pellicle. - interaction_type: MUTUALISM +- name: Proposed Yeast-Acetobacter Metabolic Cooperation + description: HYPOTHESIZED - the source describes bacteria-yeast metabolic cooperation as the general KMC model. + KMC-IMBG1 cultures contain acetobacteria and yeasts, but this composition/microscopy study does not selectively + demonstrate exchanged metabolites or reciprocal growth benefits. The four named core genera represent a larger, + condition-dependent assemblage, not an exhaustive identical membership in every culture. scope: COMMUNITY_LEVEL biological_processes: - preferred_term: fermentation @@ -120,62 +121,116 @@ ecological_interactions: label: biological process involved in interspecies interaction between organisms evidence: - reference: PMID:26061774 - supports: SUPPORT + supports: PARTIAL evidence_source: IN_VITRO snippet: mutualistic metabolic cooperation of pro- and eukaryotic microorganisms - explanation: Supports bacteria-yeast mutualistic metabolic cooperation in KMC. -- name: Cellulose Pellicle Community Structuring - description: Cellulose-producing acetobacteria create a surface pellicle that encloses the mixed yeast - and bacterial community, physically structuring biochemical interactions. - interaction_type: COLONIZATION_FACILITATION - scope: COMMUNITY_LEVEL - source_taxon: - preferred_term: Komagataeibacter + explanation: Introductory cooperation model, not a partner-removal, rescue or reciprocal-benefit experiment + in this study. + participating_taxa: + - preferred_term: Komagataeibacter term: id: NCBITaxon:1434011 label: Komagataeibacter + - preferred_term: Gluconobacter + term: + id: NCBITaxon:441 + label: Gluconobacter + - preferred_term: Pichia + term: + id: NCBITaxon:4919 + label: Pichia + - preferred_term: Brettanomyces + term: + id: NCBITaxon:13366 + label: Brettanomyces +- name: Cellulose Pellicle Community Structuring + description: Cellulose-producing bacteria form the surface pellicle, and microscopy reports mixed bacterial and + yeast morphotypes associated with its cellulose web. This preserves physical community structuring. PARTIAL + - the proposed facilitation of biochemical cooperation is distinct from observed association and was not selectively + tested by disrupting the pellicle. + scope: COMMUNITY_LEVEL biological_processes: - preferred_term: biofilm formation term: id: GO:0042710 label: biofilm formation downstream: - - target: Yeast-Acetobacter Mutualistic Fermentation - description: The cellulose pellicle encloses the mixed yeast-acetobacter - culture at the liquid surface, facilitating the close biochemical - interplay that maintains kombucha fermentation. + - target: Proposed Yeast-Acetobacter Metabolic Cooperation + description: HYPOTHESIZED - pellicle enclosure could facilitate bacteria-yeast biochemical cooperation through + spatial association, as explicitly proposed in the source Introduction. Microscopy supports association, not + a selective test that pellicle formation mediates reciprocal benefit. evidence: - reference: doi:10.1186/s13568-015-0124-5 supports: SUPPORT evidence_source: IN_VITRO snippet: cellulose-based pellicles created by the cellulose-producing bacteria - explanation: Supports acetobacterial cellulose pellicles as a structuring feature of the community. -- name: Growth-Condition Dependent Accessory Recruitment - description: KMC-IMBG1 maintained a stable acetobacterial core but shifted accessory bacteria and yeast - composition across sterile sweetened tea, nonsterile sweetened tea, honey-supplemented tea, and - cabbage-brine hybrid microenvironments. - interaction_type: NICHE_PARTITIONING + explanation: Supports cellulose pellicle formation and community structuring, not demonstrated reciprocal growth + benefits. + - reference: PMID:26061774 + supports: PARTIAL + evidence_source: IN_VITRO + snippet: Close biochemical interplay between yeasts and bacteria was facilitated by enclosing the mixed community + within cellulose-based pellicles + explanation: Source-explicit introductory spatial-facilitation model supporting the retained hypothetical arrow; + not a selective mediation experiment. + participating_taxa: + - preferred_term: Komagataeibacter + term: + id: NCBITaxon:1434011 + label: Komagataeibacter + - preferred_term: Gluconobacter + term: + id: NCBITaxon:441 + label: Gluconobacter + - preferred_term: Pichia + term: + id: NCBITaxon:4919 + label: Pichia + - preferred_term: Brettanomyces + term: + id: NCBITaxon:13366 + label: Brettanomyces +- name: Growth-Condition Dependent Community Composition + description: Culture and 16S/ITS profiles change across sterile or nonsterile sweetened tea, honey tea and the + cabbage-brine hybrid, with relatively stable acetobacterial membership and more variable yeast composition. + Lactobacilli likely introduced with cabbage brine persisted through passages. PARTIAL - active recruitment and + added fermentative capacity remain proposed; relative sequence profiles do not establish absolute growth, niche + partitioning or reciprocal benefit. scope: COMMUNITY_LEVEL biological_processes: - preferred_term: biological process involved in interspecies interaction between organisms term: id: GO:0044419 label: biological process involved in interspecies interaction between organisms - downstream: - - target: Yeast-Acetobacter Mutualistic Fermentation - description: Growth-condition shifts can recruit accessory bacteria such as - lactobacilli that may contribute to the broader KMC fermentative capacity. evidence: - reference: PMID:26061774 supports: SUPPORT evidence_source: IN_VITRO snippet: The community composition was dependent on the growth conditions - explanation: Supports growth-condition-dependent shifts in KMC composition. + explanation: Supports condition-associated community composition, not demonstrated ecological niche partitioning. - reference: PMID:26061774 supports: SUPPORT evidence_source: IN_VITRO snippet: bacterial core component in nsBTS remained the same - explanation: Supports a relatively stable bacterial core despite growth-condition changes. + explanation: Supports bacterial core continuity in nsBTS, not identical abundance or a fixed complete roster + across all conditions. + participating_taxa: + - preferred_term: Komagataeibacter + term: + id: NCBITaxon:1434011 + label: Komagataeibacter + - preferred_term: Gluconobacter + term: + id: NCBITaxon:441 + label: Gluconobacter + - preferred_term: Pichia + term: + id: NCBITaxon:4919 + label: Pichia + - preferred_term: Brettanomyces + term: + id: NCBITaxon:13366 + label: Brettanomyces cultivation_setup: - cultivation_mode: BATCH operating_temperature: 28.0 @@ -356,3 +411,29 @@ related_ingredients: snippet: for people with lactose intolerance explanation: Names lactose in the context of kombucha as a non-dairy substitute for the intolerant. metal_relevance: NOT_APPLICABLE +discussions: +- discussion_id: kmc_structure_composition_and_cooperation + prompt: Which exchanges and benefits are mediated by the KMC pellicle or accessory members? + kind: KNOWLEDGE_GAP + status: OPEN + attaches_to: + - ecological_interactions#Proposed Yeast-Acetobacter Metabolic Cooperation + - ecological_interactions#Cellulose Pellicle Community Structuring + - ecological_interactions#Growth-Condition Dependent Community Composition + rationale: 'Retain the source-explicit pellicle-to-cooperation model as HYPOTHESIZED, with physical association + distinct from selective mediation. Remove the accessory-composition-to-mutualism arrow: possible fermentation + capacity does not establish reciprocal benefit among the canonical core genera. Positive pellicle formation, + composition changes and persistence of probable cabbage-derived lactobacilli remain represented. Read the full + primary main text and main/supplement captions, not original figure pixels, Table 1 numerical cells, supplementary + PDF or raw sequences. Strain-resolved transfer and selective pellicle/accessory perturbations remain open questions. + Canonical membership is preserved as a partial core representation; separate non-graph cultivation/ingredient + claims remain unresolved under #1543.' + posed_by: codex + posed_date: '2026-10-06' +curation_history: +- timestamp: '2026-10-06T20:19:10Z' + curator: codex + action: REVIEW_CAUSAL_GRAPH + changes: 'Qualify KMC pellicle cooperation and remove the unsupported recruitment-to-mutualism arrow. Issue #1539; + reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml' + llm_assisted: true diff --git a/reports/causal_graph_review/README.md b/reports/causal_graph_review/README.md index dcf93d58b..921e80a4e 100644 --- a/reports/causal_graph_review/README.md +++ b/reports/causal_graph_review/README.md @@ -5,6 +5,27 @@ source-based self-adversarial review. These records are not independent approval or proof that every graph is complete. The current inventory and summary distinguish `reviewed`, `pending` and `needs_research`. +## Batch60: KZ, KMC And Co-inducible Biosynthesis + +[Decisions](decisions/20261006-kz-kmc-komagataella-batch60.yaml), +[validation](validation-20261006-batch60.json) and +[self-adversarial review](adversarial-review-20261006-batch60.yaml) account for +four original nodes and two arrows, plus the empty Komagataella-E. coli graph. +KZ retains positive outcomes and assay-inferred complementary roles without +asserting directed strain transfer. Its unread corrigendum and complete mechanism +verification remain `needs_research` under #1541. + +KMC retains all three nodes and one source-explicit pellicle/cooperation arrow as +`HYPOTHESIZED`; accessory composition is not established reciprocal benefit. +Its full primary main text and captions were read, not original images, numerical +Table 1 cells or supplementary PDF. Non-graph metadata #1543 remains open. + +Komagataella remains `needs_research` under #1542. Its reviewed one-community +Edison dry run contains 7,457 characters, with no paid submission or credits. +The public primary preview is not complete Methods or verified strain/pathway +assignment. All 920 reference caches are unchanged. Repairs #1539 and #1540 await +protected-main merge; the research and metadata issues stay open until resolved. + ## Batch59: Kefir Flavor And Dairy-Panel Gap [Decisions](decisions/20261006-kefir-dairy-batch59.yaml), diff --git a/reports/causal_graph_review/adversarial-review-20261006-batch60.yaml b/reports/causal_graph_review/adversarial-review-20261006-batch60.yaml new file mode 100644 index 000000000..2fa7a23cf --- /dev/null +++ b/reports/causal_graph_review/adversarial-review-20261006-batch60.yaml @@ -0,0 +1,179 @@ +reviewer: codex +review_mode: source_based_self_adversarial_review +independent_approval: false +base_commit: 4aed48731d913b28b88cd42e5bf8e425f4c8c2d9 +scope: +- kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml +- kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml +- kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml +decision_ledger: reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml +validation_report: reports/causal_graph_review/validation-20261006-batch60.json +findings: +- issue: 1539 + severity: P2 + status: addressed_in_this_change + finding: KMC pellicle model versus observed structure and unsupported recruitment endpoint. + disposition: Close only after protected-main merge. +- issue: 1540 + severity: P2 + status: addressed_in_this_change + finding: KZ assay roles encoded as resolved pairwise transfer. + disposition: Close only after protected-main merge. +- issue: 1541 + severity: P2 + status: unresolved_research + finding: KZ corrigendum and complete mechanism verification. + disposition: Keep OPEN; needs_research. +- issue: 1542 + severity: P2 + status: unresolved_research + finding: Komagataella empty causal graph and exact pathway/chassis evidence. + disposition: Keep OPEN; needs_research. +- issue: 1543 + severity: P2 + status: unresolved_non_graph + finding: KMC cultivation and ingredient metadata. + disposition: Keep OPEN after graph repair merge. +adversarial_checks: +- Whole three records read; all four original nodes and both arrows assessed, plus the absent Komagataella + interaction field. Missing structure is not certified absence of biology. +- KZ retains positive consortium outcomes and complementary biomass-normalized assays. Community-level + scope with both exact canonical strain labels removes unverified SZ-3-to-CW-D3T transfer direction without + erasing the division-of-labor interpretation. No new node or arrow. +- KZ full Methods and linked correction body remain unread. PubMed identifies PMID41456976; official publisher + API returns core metadata only and openaccessArticle=false. Third-party OA labels do not establish access + or scientific correction content. Keep needs_research under1541; do not assume the correction is immaterial. +- KMC source Introduction explicitly states pellicle-supported biochemical interplay. Retain that existing + arrow as HYPOTHESIZED, grounded by a primary-matched excerpt, while distinguishing observed pellicle/morphotype + association from selective mediation of reciprocal benefit. +- Remove the accessory-composition-to-mutualism arrow because potential fermentation capacity is not measured + reciprocal benefit among the four core genera. Preserve positive composition changes and probable cabbage-derived + lactobacillus persistence; do not convert relative read proportions into absolute growth or niche partitioning. +- KMC four canonical genera are a partial core representation, not every organism in every treatment. + No canonical taxonomy edit. Non-graph assertions about separately cultivating members, categorical shaking + effects and ingredients remain separately unresolved under1543. +- Komagataella public abstract and publisher Introduction/section previews report compatible co-growth, + common induction and biosynthetic relays. Lack of engineered population-balancing crosstalk is not biological + neutralism; common induction is not cross-feeding. Exact host/construct assignments and full Methods + remain unresolved; do not borrow unmatched monoculture titres as experimental controls. +- Komagataella stays needs_research under1542, with zero nodes/arrows and its originally absent interaction + field preserved. The reviewed one-community LITERATURE dry-run query contains7457 characters; zero provider + submissions or credits. No report or completed extension is claimed. +- KMC full extracted primary main text and main/supplement captions were read. Original figure pixels, + Table1 numerical cells, supplementary PDF and raw sequences were not inspected. KZ/Komagataella complete + abstracts plus selected public publisher previews were read, not complete primary bodies. No access-control + workaround. +- Full biological diffs, exact participant registry and focused test assertions reviewed.457 other record + hashes and920 cache hashes unchanged; raw non-graph blocks preserved. Both-root census explicitly measures + KZ scope change, with no auditor-rule edit.36 network warnings preserved and zero structural defects. +- Actual per-record schema/strict/term/reference/snippet/rank/history gates and regression outputs/digests + recorded. Repeat renders and456-page exact description parser passed, not browser screenshots. Post-commit + docs-current is a separate publication gate. +- 839 all-state issue titles/bodies and19 scoped ignored/hidden local matches searched; all three matching + issue bodies read, not every comment/PR or local file. Repair1539/1540 addressed but OPEN until protected-main + merge; research1541/1542 and metadata1543 remain unresolved. Exact-head self-review is COMMENT, not + independent approval. +verification: + combined_regressions: 552 passed in 188.20s (0:03:08) + new_regression_tests: 8 + schema_strict_terms_history: Passed with actual output digests + references: CLI Total checks counts reported issues, not executed checks. Cache MATCH does not prove + scientific entailment or complete provenance. All920 caches unchanged. KMC retained-arrow fragment + independently matched to fresh publisher primary text. KZ correction body and full mechanism1541, + empty Komagataella graph1542 and KMC non-graph1543 remain unresolved. Existing476/1091/1095 limitations + remain. + snippet_counts_by_record: + kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml: + MATCH: 13 + RENDERING: 0 + ASSEMBLED: 0 + WEAK: 0 + MISMATCH: 0 + NOCONTENT: 0 + kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml: + MATCH: 22 + RENDERING: 0 + ASSEMBLED: 0 + WEAK: 0 + MISMATCH: 0 + NOCONTENT: 0 + kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml: + MATCH: 7 + RENDERING: 0 + ASSEMBLED: 0 + WEAK: 0 + MISMATCH: 0 + NOCONTENT: 0 + network: + existing_findings_preserved: 36 + added_findings: {} + removed_findings: {} + black_ruff_diff: passed + tested_file_sha256: + reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml: 1e64150e4f7f3cde4b39dfc6bd97f2984ac3fcf26486b31bdbcdc710e2874a69 + tests/test_causal_review_batch60.py: dd1e198d098c1fb74896d4820253859100932c708930bd55c287af99174e2133 + tests/test_participating_taxa.py: 27d2df501648e404a9d722844d513ebb29719034fc911348055d161842bf4a02 + kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml: 65275177a514ceb42276f4f416da7b727e5200b3a8f79fb1510cf6c49bbd083c + kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml: 9df19ae1add5269721799ee3a8328edf0531e314c15e3a3abc71a2e4e659fa40 + kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml: f4024c9731b98eac233911893d44568c3a9097460fb1aa3fb93fced0647cb746 + reports/causal_graph_review/README.md: 872013dbfec5086ef9db848b4f3d3578c68366efe4c5a7523f641bb9d50e0bf9 + unchanged_caches: 920 + edison_dry_run: + path: research/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-edison-literature-causal-meta.yaml + sha256: 753bcec2844b7256e2790a9907e2c2e2d0d18d3a01fe3d39dbd9d79db395b328 + query_sha256: e429f0f613ac80c47e2e8ddfd83510abb8f87ba13c495f0d19a087207a5c33bb + query_chars: 7457 + job: LITERATURE + status: dry-run + provider_submissions: 0 + credits_spent: 0 + paid_authorization: specific one-job request pending + post_commit_docs_current: Separate publication gate, not asserted before commit. +participant_census: + measurements: + baseline: + records: 460 + with_community_level: 390 + mixed: 124 + community_level_only: 266 + taxa: 1505 + credited_solely_by_the_rule: 1236 + current: + records: 460 + with_community_level: 391 + mixed: 124 + community_level_only: 267 + taxa: 1507 + credited_solely_by_the_rule: 1238 + interpretation: KZ changes one pairwise interpretation to an explicitly two-member community-level observation; + KMC narrows all three nodes to its four canonical core genera. Canonical taxonomy is unchanged. Census + CL records390 to391, CL-only266 to267, taxa1505 to1507 and solely credited1236 to1238; no auditor-rule + change. +corpus: + records: 460 + nodes: 1458 + edges: 651 + review_status_counts: + needs_research: 21 + pending: 245 + reviewed: 194 +limitations: +- All four original nodes and two arrows assessed; one explicit source model retained as HYPOTHESIZED + and one unsupported endpoint removed. No new causal structure. +- KZ positive performance and assay-inferred roles preserved. Primary full Methods/supplements and correction + body unavailable; needs_research under1541. Publisher API metadata contradicts third-party OA labels; + no access-control bypass. +- KMC full primary main text and main/supplement captions read. Numerical Table 1 cells, original figures, + supplementary PDF and raw data not inspected. Cooperation is a source model, not demonstrated reciprocal + mediation. +- Komagataella abstract and public publisher Introduction/section previews read, not full Methods/supplements. + Empty graph remains needs_research under1542; no new nodes/arrows. One reviewed 7457-character Edison + dry run, zero provider submissions or credits. +- Canonical taxa and raw non-graph blocks preserved, not recertified. KMC metadata1543 and research1541/1542 + remain OPEN after graph repair merge. +- All920 reference caches unchanged. Primary-matched graph fragment does not certify full historical cache + provenance or SupportingReference coverage476; Linux DOI lookup1091 and writer artifact1095 remain limitations. +- Deduplication searched839 all-state issue titles/bodies and19 scoped ignored/hidden local matches; all + three matching issue bodies read, not every comment/PR or local match. +- Self-adversarial review is COMMENT, not independent approval. Repair1539/1540 remain OPEN until protected-main + merge. diff --git a/reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml b/reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml new file mode 100644 index 000000000..35dbf1802 --- /dev/null +++ b/reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml @@ -0,0 +1,206 @@ +reviewer: codex +review_mode: source_based_self_adversarial_review +independent_approval: false +base_commit: 4aed48731d913b28b88cd42e5bf8e425f4c8c2d9 +baseline_commit: 4aed48731d913b28b88cd42e5bf8e425f4c8c2d9 +base_tree: 06928b0ddcfb76a9d528a964943f9c9e8786cf41 +pre_source_baseline_sha256: ab4d9c40428b46f7bb6cc997595de77fbf97ec46189ca332940edecc90774bbf +issues: +- 1539 +- 1540 +unresolved_non_graph_issues: +- 1543 +unresolved_research_issues: +- 1541 +- 1542 +issue_deduplication: + all_state_issue_titles_and_bodies: 839 + ignored_hidden_local_search: true + matching_local_files: 19 + matching_issues: + - 1091 + - 595 + - 594 +reference_cache_original_hashes: + references_cache/PMID_41365368.txt: 181b208bcf0f0961381d266a85ae94398ce40dfada4e0885c1a80a3b882ff900 + references_cache/PMID_42492893.txt: 8e2d768ba6c299346f4e718a57ee8b69fdb9a09769bbb13ba9dd2d034f4cb015 + references_cache/PMID_26061774.md: 41972c5446b8dabb15959a06bd15acefab8c640c60b9feb415e444d7c6793b4f + references_cache/PMID_26061774.txt: 8df7889382fec72eb87e399fd890913cb4f0d330a59fd31a4638def08af61828 + references_cache/doi_10.1186_s13568-015-0124-5.txt: 24ea6e18e130e868a17112d4503e7d9b7a2337a7bb8501662e21df88a336ec09 + references_cache/europepmc_10.1186_s13568-015-0124-5.txt: 6e68b9fda9968ec36956406ff5205930f7a3eab47e57e7320d90244debeeae4f +primary_artifacts: + /private/tmp/communitymech-batch60/kombucha-primary.html: d9510aedb4e415469d8568c4474b0f64f8f4dac15c8f58ef41336be82ee3be56 + /private/tmp/communitymech-batch60/kombucha-primary.txt: 7fd7c46845afdfea0743fd39b6f10ee06f8455153f81f70d361d96d242704315 + /private/tmp/communitymech-batch60/kz-indexed-primary.txt: ee5dc9e5e44dd3d9f49d340b103f8552f9649d1473832c34791f53409abb3b35 + /private/tmp/communitymech-batch60/kz-correction-crossref.json: b76e5ed2cca7b4fe4e2b437ef281802c132589686d470f38b9f6ca05696e555b + /private/tmp/communitymech-batch60/kz-correction-primary.xml: 96f5611f4df555213ab98248c778172ada028c941e5cdee3b067000cdb4886ec + /private/tmp/communitymech-batch60/komagataella-indexed-primary.txt: 76a968c26f974b084b3b61102f6dcda072a308615999e3144d2cc66aad8c517b +primary_urls: +- https://link.springer.com/article/10.1186/s13568-015-0124-5 +- https://pubmed.ncbi.nlm.nih.gov/41365368/ +- https://pubmed.ncbi.nlm.nih.gov/41456976/ +- https://www.sciencedirect.com/science/article/pii/S0960852425017493 +- https://www.sciencedirect.com/science/article/pii/S1096717626001023 +primary_matched_fragment: Close biochemical interplay between yeasts and bacteria was facilitated by enclosing the + mixed community within cellulose-based pellicles +edison_dry_run: + path: research/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-edison-literature-causal-meta.yaml + sha256: 753bcec2844b7256e2790a9907e2c2e2d0d18d3a01fe3d39dbd9d79db395b328 + query_sha256: e429f0f613ac80c47e2e8ddfd83510abb8f87ba13c495f0d19a087207a5c33bb + query_chars: 7457 + job: LITERATURE + status: dry-run + provider_submissions: 0 + credits_spent: 0 + paid_authorization: specific one-job request pending +limitations: +- All four original nodes and two arrows assessed; one explicit source model retained as HYPOTHESIZED and one unsupported + endpoint removed. No new causal structure. +- KZ positive performance and assay-inferred roles preserved. Primary full Methods/supplements and correction body + unavailable; needs_research under1541. Publisher API metadata contradicts third-party OA labels; no access-control + bypass. +- KMC full primary main text and main/supplement captions read. Numerical Table 1 cells, original figures, supplementary + PDF and raw data not inspected. Cooperation is a source model, not demonstrated reciprocal mediation. +- Komagataella abstract and public publisher Introduction/section previews read, not full Methods/supplements. Empty + graph remains needs_research under1542; no new nodes/arrows. One reviewed 7457-character Edison dry run, zero + provider submissions or credits. +- Canonical taxa and raw non-graph blocks preserved, not recertified. KMC metadata1543 and research1541/1542 remain + OPEN after graph repair merge. +- All920 reference caches unchanged. Primary-matched graph fragment does not certify full historical cache provenance + or SupportingReference coverage476; Linux DOI lookup1091 and writer artifact1095 remain limitations. +- Deduplication searched839 all-state issue titles/bodies and19 scoped ignored/hidden local matches; all three matching + issue bodies read, not every comment/PR or local match. +- Self-adversarial review is COMMENT, not independent approval. Repair1539/1540 remain OPEN until protected-main + merge. +records: +- path: kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml + id: CommunityMech:000344 + original_sha256: 1d9b711fa86c876ec1657a114242ebba6cb14cd8be5323a25baeae6511c9b520 + record_sha256: 65275177a514ceb42276f4f416da7b727e5200b3a8f79fb1510cf6c49bbd083c + status: needs_research + outcome: changed + review_file: reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml + scope: Whole record and every original node/arrow, including empty-graph assessment. + summary: Bound KZ catabolic roles to the reported assays and flag unresolved corrigendum verification. + renamed_nodes: + SZ-3 to CW-D3T phenanthrene-mineralization division of labor: Assay-Inferred Phenanthrene Catabolic Division + Of Labor + node_decisions: + - node: Assay-Inferred Phenanthrene Catabolic Division Of Labor + rationale: Preserve positive KZ performance and complementary catalytic assays, remove unverified pairwise direction, + and keep correction/mechanistic verification pending. + removed_node_decisions: [] + edges_before: [] + edges_after: [] + retained_edge_decisions: [] + removed_edge_decisions: [] + references: + - PMID:41365368 + - PMID:41456976 + issues: + - 1540 + - 1541 + allowed_changed_fields: + - curation_history + - discussions + - ecological_interactions + curation_events_added: 1 + history_files: + - history/records/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom/2026-10-06T201911Z-codex-4edaec.yaml + source_review: + scope: Complete abstract and public publisher Introduction/section previews; original full Methods, figures, + supplements and correction body unread. Official correction API metadata only. + cache_provenance_certified: false +- path: kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml + id: CommunityMech:000154 + original_sha256: 774000556eeff1dbbdb5e1890c5de7d6ee0f0f9e56e40973f2e40b5c61d06960 + record_sha256: 9df19ae1add5269721799ee3a8328edf0531e314c15e3a3abc71a2e4e659fa40 + status: reviewed + outcome: changed + review_file: reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml + scope: Whole record and every original node/arrow, including empty-graph assessment. + summary: Qualify KMC pellicle cooperation and remove the unsupported recruitment-to-mutualism arrow. + renamed_nodes: + Yeast-Acetobacter Mutualistic Fermentation: Proposed Yeast-Acetobacter Metabolic Cooperation + Growth-Condition Dependent Accessory Recruitment: Growth-Condition Dependent Community Composition + node_decisions: + - node: Proposed Yeast-Acetobacter Metabolic Cooperation + rationale: Retain the authors' cooperation model as hypothetical, not measured reciprocal benefit. + - node: Cellulose Pellicle Community Structuring + rationale: Retain observed pellicle structuring and the explicitly hypothetical spatial-facilitation arrow. + - node: Growth-Condition Dependent Community Composition + rationale: Retain condition-associated composition and accessory persistence without niche-partitioning or mutualism + inference. + removed_node_decisions: [] + edges_before: + - source: Cellulose Pellicle Community Structuring + target: Yeast-Acetobacter Mutualistic Fermentation + description: The cellulose pellicle encloses the mixed yeast-acetobacter culture at the liquid surface, facilitating + the close biochemical interplay that maintains kombucha fermentation. + - source: Growth-Condition Dependent Accessory Recruitment + target: Yeast-Acetobacter Mutualistic Fermentation + description: Growth-condition shifts can recruit accessory bacteria such as lactobacilli that may contribute + to the broader KMC fermentative capacity. + edges_after: + - source: Cellulose Pellicle Community Structuring + target: Proposed Yeast-Acetobacter Metabolic Cooperation + description: HYPOTHESIZED - pellicle enclosure could facilitate bacteria-yeast biochemical cooperation through + spatial association, as explicitly proposed in the source Introduction. Microscopy supports association, not + a selective test that pellicle formation mediates reciprocal benefit. + retained_edge_decisions: + - source: Cellulose Pellicle Community Structuring + target: Proposed Yeast-Acetobacter Metabolic Cooperation + rationale: The source explicitly states spatial facilitation; retain the existing arrow as HYPOTHESIZED, not + experimentally isolated mediation. + removed_edge_decisions: + - source: Growth-Condition Dependent Accessory Recruitment + target: Yeast-Acetobacter Mutualistic Fermentation + rationale: Possible accessory fermentation capacity does not establish reciprocal benefit among the canonical + core genera; preserve the hypothesis in prose, not this causal endpoint. + references: + - PMID:26061774 + - doi:10.1186/s13568-015-0124-5 + issues: + - 1539 + - 1543 + allowed_changed_fields: + - curation_history + - discussions + - ecological_interactions + curation_events_added: 1 + history_files: + - history/records/Kombucha_KMC_IMBG1_Fermentation_Community/2026-10-06T201911Z-codex-36fc6c.yaml + source_review: + scope: Full extracted primary main text, Methods, Results, Discussion, main captions and supplement caption. + Table 1 numerical cells, original images, supplementary PDF and raw sequences not inspected. + cache_provenance_certified: false +- path: kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml + id: CommunityMech:000343 + original_sha256: e07dcd6b1d7bdfeb955aaca0f93df3aadc7f36b4e83f5869974a44e76c6232d2 + record_sha256: f4024c9731b98eac233911893d44568c3a9097460fb1aa3fb93fced0647cb746 + status: needs_research + outcome: changed + review_file: reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml + scope: Whole record and every original node/arrow, including empty-graph assessment. + summary: Expose the unresolved empty Komagataella-E. coli causal biosynthesis graph. + renamed_nodes: {} + node_decisions: [] + removed_node_decisions: [] + edges_before: [] + edges_after: [] + retained_edge_decisions: [] + removed_edge_decisions: [] + references: + - PMID:42492893 + issues: + - 1542 + allowed_changed_fields: + - curation_history + - discussions + curation_events_added: 1 + history_files: + - history/records/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture/2026-10-06T201911Z-codex-e2d63d.yaml + source_review: + scope: Complete abstract and public publisher Introduction/section previews. Full Methods, original figures, + supplements and construct assignments not inspected. + cache_provenance_certified: false diff --git a/reports/causal_graph_review/inventory.tsv b/reports/causal_graph_review/inventory.tsv index 11028b690..824d8ceaa 100644 --- a/reports/causal_graph_review/inventory.tsv +++ b/reports/causal_graph_review/inventory.tsv @@ -211,9 +211,9 @@ kb/communities/KBase_Models_for_Zahmeeth_Original_PLOS.yaml CommunityMech:000037 kb/communities/KBase_ORT_Workflow_Community_Model.yaml CommunityMech:000038 b4cfa6d608db1e2d04f70537c425d23def58fb2528394a9226b82f4cc609ebf2 3 2 90.0 reports/causal_graph_review/decisions/20261006-three-records-batch56.yaml reviewed kb/communities/KBase_Synthetic_Bacterial_Community_R2A.yaml CommunityMech:000039 8239899f70763acf1aa6be423b37e77737a7e6367b6203888c96e163e9419753 2 0 65.0 reports/causal_graph_review/decisions/20261006-r2a-batch58.yaml reviewed kb/communities/Kefir_Flavor_Lentilactobacillus_Kluyveromyces_Coculture.yaml CommunityMech:000332 f562c28689b45ec3c53061db80bb3c6f33c4e464fe82b7d2d8236eebf53c383d 4 0 65.0 reports/causal_graph_review/decisions/20261006-kefir-dairy-batch59.yaml reviewed -kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml CommunityMech:000344 1d9b711fa86c876ec1657a114242ebba6cb14cd8be5323a25baeae6511c9b520 1 0 65.0 pending -kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml CommunityMech:000343 e07dcd6b1d7bdfeb955aaca0f93df3aadc7f36b4e83f5869974a44e76c6232d2 0 0 0.0 pending -kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml CommunityMech:000154 774000556eeff1dbbdb5e1890c5de7d6ee0f0f9e56e40973f2e40b5c61d06960 3 2 100.0 pending +kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml CommunityMech:000344 65275177a514ceb42276f4f416da7b727e5200b3a8f79fb1510cf6c49bbd083c 1 0 55.0 reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml needs_research +kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml CommunityMech:000343 f4024c9731b98eac233911893d44568c3a9097460fb1aa3fb93fced0647cb746 0 0 0.0 reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml needs_research +kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml CommunityMech:000154 9df19ae1add5269721799ee3a8328edf0531e314c15e3a3abc71a2e4e659fa40 3 1 80.0 reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml reviewed kb/communities/LBNL_Brachypodium_Drought_SynCom15.yaml CommunityMech:000088 0b4d516a93f86c8a477d6054c37966acda52632cb36846bce9be93bc235851fb 7 0 75.0 pending kb/communities/LBNL_Human_Gut_Interaction_SynCom.yaml CommunityMech:000122 3372341a0b7ba1efb0a2d84070e70716f5940225c9f5fbee44f5427e777f7744 3 2 100.0 pending kb/communities/LBNL_Switchgrass_Soil_SynCom16.yaml CommunityMech:000087 2c5d49aaf802b1455416362350dfe4e62dff6e3c461e31166a8b85d3e0b3eaa0 5 0 75.0 pending diff --git a/reports/causal_graph_review/summary.json b/reports/causal_graph_review/summary.json index da34c0452..5caca5b7b 100644 --- a/reports/causal_graph_review/summary.json +++ b/reports/causal_graph_review/summary.json @@ -3,13 +3,13 @@ "semantic_review_rule": "Structural checks and readiness scores do not establish causal support. Completed reviews must match the current record SHA-256, cover every node once with a rationale, and reproduce the current edge list.", "records": 460, "nodes": 1458, - "edges": 652, + "edges": 651, "records_without_nodes": 8, "records_without_edges": 156, "review_status_counts": { - "needs_research": 19, - "pending": 248, - "reviewed": 193 + "needs_research": 21, + "pending": 245, + "reviewed": 194 }, "structural_defects": [], "network_audit": [ diff --git a/reports/causal_graph_review/validation-20261006-batch60.json b/reports/causal_graph_review/validation-20261006-batch60.json new file mode 100644 index 000000000..057f37368 --- /dev/null +++ b/reports/causal_graph_review/validation-20261006-batch60.json @@ -0,0 +1,814 @@ +{ + "base_commit": "4aed48731d913b28b88cd42e5bf8e425f4c8c2d9", + "scope": [ + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml", + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml", + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml" + ], + "reference_validator_version": "0.1.7", + "results": [ + { + "check": "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-schema", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-validate", + "-s", + "src/communitymech/schema/communitymech.yaml", + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-schema.log", + "log_sha256": "de6087f4be8c0adc015333597cd864406ad889bbde42d9ee20db0df5932bb2de", + "output_tail": "No issues found\n" + }, + { + "check": "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-strict", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/neDBQyZXv0RYv8B1GH0dI/bin/python3", + "scripts/validate_strict.py", + "--workers", + "1", + "--out", + "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-strict.tsv", + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-strict.log", + "log_sha256": "ac67592eb7641779eba8d5931eecdcc19f6d2bb84b146feb497b32c166ba1519", + "output_tail": "Validating 1 files with 1 workers; schema=/private/tmp/communitymech-causal-batch60/src/communitymech/schema/communitymech.yaml\n\n=== validate-strict summary ===\n files scanned: 1\n files with ERROR: 0\n total ERROR rows: 0\n TSV: /private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-strict.tsv\n" + }, + { + "check": "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-terms", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/neDBQyZXv0RYv8B1GH0dI/bin/linkml-term-validator", + "validate-data", + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml", + "-s", + "src/communitymech/schema/communitymech.yaml", + "--labels" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-terms.log", + "log_sha256": "23acda1c1d3194d12bc947d897d07de83f4a076a9d93c135148885108530e018", + "output_tail": "\u2705 Validation passed\n" + }, + { + "check": "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-references", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-reference-validator", + "validate", + "data", + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml", + "-s", + "src/communitymech/schema/communitymech.yaml", + "--config", + "conf/reference_validator.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-references.log", + "log_sha256": "b567f443f551b8e8c1f5d2e8c1a00d80a41d760572857c520306f9d447f102cd", + "output_tail": "Validating kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml against schema src/communitymech/schema/communitymech.yaml\nCache directory: references_cache\n\nValidation Summary:\n Total checks: 0\n All validations passed!\n", + "explained_output_tail": "Validating kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml against schema src/communitymech/schema/communitymech.yaml\nCache directory: references_cache\n\nValidation Summary:\n Total checks: 0\n All validations passed!\n" + }, + { + "check": "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-snippets", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "scripts/evidence_snippet_audit.py", + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml", + "--list-mismatch", + "--list-nocontent", + "--list-rendering", + "--list-assembled" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-snippets.log", + "log_sha256": "6fd22c7b42f139e3b66a27210ad3d8968c80af940879c7074184305812838cca", + "output_tail": "# 13 evidence snippets scanned across 1 files\n MATCH 13\n RENDERING 0\n ASSEMBLED 0\n WEAK 0\n MISMATCH 0\n NOCONTENT 0\n\n# Files with MISMATCH/WEAK (content present but snippet absent) \u2014 fabrication suspects\n\n# MISMATCH/WEAK detail\n\n# Files by RENDERING (faithful quote; differs from cache only by\n# punctuation/whitespace/Greek \u2014 this is what validate-references flags)\n\n# Top files by NOCONTENT (unverifiable; cache stub/missing)\n\n# ASSEMBLED (every part is in the source; the join is not \u2014\n# a table flattened into prose, or a quote welded from two places)\n" + }, + { + "check": "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-rank", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "scripts/rank_causal_graph_readiness.py", + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml", + "--format", + "json" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-rank.log", + "log_sha256": "f6429256a145c02998319fdf6e312f6045c873ea6b6a7ef4cbd08b1f4830f9cd", + "output_tail": "[\n {\n \"rank\": 1,\n \"id\": \"CommunityMech:000344\",\n \"name\": \"Klebsiella-Arthrobacter KZ Phenanthrene-Cadmium SynCom\",\n \"score\": 55.0,\n \"taxonomy\": 2,\n \"connected_taxa\": 2,\n \"disconnected_taxa\": 0,\n \"ecological_interactions\": 1,\n \"downstream_edges\": 0,\n \"interaction_evidence\": 2,\n \"dangling_downstream\": 0,\n \"self_downstream\": 0,\n \"missing\": [\n \"additional_interactions\",\n \"downstream\",\n \"interaction_type\"\n ],\n \"path\": \"kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml\"\n }\n]\n" + }, + { + "check": "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-history-0", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-validate", + "-s", + "src/communitymech/schema/history.yaml", + "--target-class", + "HistoryRecord", + "history/records/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom/2026-10-06T201911Z-codex-4edaec.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom-history-0.log", + "log_sha256": "de6087f4be8c0adc015333597cd864406ad889bbde42d9ee20db0df5932bb2de", + "output_tail": "No issues found\n" + }, + { + "check": "Kombucha_KMC_IMBG1_Fermentation_Community-schema", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-validate", + "-s", + "src/communitymech/schema/communitymech.yaml", + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-schema.log", + "log_sha256": "de6087f4be8c0adc015333597cd864406ad889bbde42d9ee20db0df5932bb2de", + "output_tail": "No issues found\n" + }, + { + "check": "Kombucha_KMC_IMBG1_Fermentation_Community-strict", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/neDBQyZXv0RYv8B1GH0dI/bin/python3", + "scripts/validate_strict.py", + "--workers", + "1", + "--out", + "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-strict.tsv", + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-strict.log", + "log_sha256": "44d905818ea60d437d582107b10f5253880553c5333e2860258975b89b40cebd", + "output_tail": "Validating 1 files with 1 workers; schema=/private/tmp/communitymech-causal-batch60/src/communitymech/schema/communitymech.yaml\n\n=== validate-strict summary ===\n files scanned: 1\n files with ERROR: 0\n total ERROR rows: 0\n TSV: /private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-strict.tsv\n" + }, + { + "check": "Kombucha_KMC_IMBG1_Fermentation_Community-terms", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/neDBQyZXv0RYv8B1GH0dI/bin/linkml-term-validator", + "validate-data", + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml", + "-s", + "src/communitymech/schema/communitymech.yaml", + "--labels" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-terms.log", + "log_sha256": "23acda1c1d3194d12bc947d897d07de83f4a076a9d93c135148885108530e018", + "output_tail": "\u2705 Validation passed\n" + }, + { + "check": "Kombucha_KMC_IMBG1_Fermentation_Community-references", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-reference-validator", + "validate", + "data", + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml", + "-s", + "src/communitymech/schema/communitymech.yaml", + "--config", + "conf/reference_validator.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-references.log", + "log_sha256": "eedce5f0cf8aa3782423e0c2199f82ccd86be485cccb947597c26762f5264cb8", + "output_tail": "Validating kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml against schema src/communitymech/schema/communitymech.yaml\nCache directory: references_cache\n\nValidation Summary:\n Total checks: 0\n All validations passed!\n", + "explained_output_tail": "Validating kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml against schema src/communitymech/schema/communitymech.yaml\nCache directory: references_cache\n\nValidation Summary:\n Total checks: 0\n All validations passed!\n" + }, + { + "check": "Kombucha_KMC_IMBG1_Fermentation_Community-snippets", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "scripts/evidence_snippet_audit.py", + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml", + "--list-mismatch", + "--list-nocontent", + "--list-rendering", + "--list-assembled" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-snippets.log", + "log_sha256": "96a23c88588d68e990af5d62f9c27df12879566b4125e7703a275c3bfa7adcd7", + "output_tail": "# 22 evidence snippets scanned across 1 files\n MATCH 22\n RENDERING 0\n ASSEMBLED 0\n WEAK 0\n MISMATCH 0\n NOCONTENT 0\n\n# Files with MISMATCH/WEAK (content present but snippet absent) \u2014 fabrication suspects\n\n# MISMATCH/WEAK detail\n\n# Files by RENDERING (faithful quote; differs from cache only by\n# punctuation/whitespace/Greek \u2014 this is what validate-references flags)\n\n# Top files by NOCONTENT (unverifiable; cache stub/missing)\n\n# ASSEMBLED (every part is in the source; the join is not \u2014\n# a table flattened into prose, or a quote welded from two places)\n" + }, + { + "check": "Kombucha_KMC_IMBG1_Fermentation_Community-rank", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "scripts/rank_causal_graph_readiness.py", + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml", + "--format", + "json" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-rank.log", + "log_sha256": "3e86f216876fbc6d91a2a3d3b645e8683976fc02d43fd75ea40d5b8c5c4a5d95", + "output_tail": "[\n {\n \"rank\": 1,\n \"id\": \"CommunityMech:000154\",\n \"name\": \"Kombucha KMC-IMBG1 Fermentation Community\",\n \"score\": 80.0,\n \"taxonomy\": 4,\n \"connected_taxa\": 4,\n \"disconnected_taxa\": 0,\n \"ecological_interactions\": 3,\n \"downstream_edges\": 1,\n \"interaction_evidence\": 5,\n \"dangling_downstream\": 0,\n \"self_downstream\": 0,\n \"missing\": [\n \"additional_downstream\",\n \"interaction_type\"\n ],\n \"path\": \"kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml\"\n }\n]\n" + }, + { + "check": "Kombucha_KMC_IMBG1_Fermentation_Community-history-0", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-validate", + "-s", + "src/communitymech/schema/history.yaml", + "--target-class", + "HistoryRecord", + "history/records/Kombucha_KMC_IMBG1_Fermentation_Community/2026-10-06T201911Z-codex-36fc6c.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Kombucha_KMC_IMBG1_Fermentation_Community-history-0.log", + "log_sha256": "de6087f4be8c0adc015333597cd864406ad889bbde42d9ee20db0df5932bb2de", + "output_tail": "No issues found\n" + }, + { + "check": "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-schema", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-validate", + "-s", + "src/communitymech/schema/communitymech.yaml", + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-schema.log", + "log_sha256": "de6087f4be8c0adc015333597cd864406ad889bbde42d9ee20db0df5932bb2de", + "output_tail": "No issues found\n" + }, + { + "check": "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-strict", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/neDBQyZXv0RYv8B1GH0dI/bin/python3", + "scripts/validate_strict.py", + "--workers", + "1", + "--out", + "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-strict.tsv", + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-strict.log", + "log_sha256": "2b15b03b063d335e76b52dfc3812077d47236b80f10343c1e40a8694b1e6cc85", + "output_tail": "Validating 1 files with 1 workers; schema=/private/tmp/communitymech-causal-batch60/src/communitymech/schema/communitymech.yaml\n\n=== validate-strict summary ===\n files scanned: 1\n files with ERROR: 0\n total ERROR rows: 0\n TSV: /private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-strict.tsv\n" + }, + { + "check": "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-terms", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/neDBQyZXv0RYv8B1GH0dI/bin/linkml-term-validator", + "validate-data", + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml", + "-s", + "src/communitymech/schema/communitymech.yaml", + "--labels" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-terms.log", + "log_sha256": "23acda1c1d3194d12bc947d897d07de83f4a076a9d93c135148885108530e018", + "output_tail": "\u2705 Validation passed\n" + }, + { + "check": "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-references", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-reference-validator", + "validate", + "data", + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml", + "-s", + "src/communitymech/schema/communitymech.yaml", + "--config", + "conf/reference_validator.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-references.log", + "log_sha256": "be2931cc389c6a7ddd8bb8cf49ddc9f6f67fa122d264246f48b5236a363e3863", + "output_tail": "Validating kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml against schema src/communitymech/schema/communitymech.yaml\nCache directory: references_cache\n\nValidation Summary:\n Total checks: 0\n All validations passed!\n", + "explained_output_tail": "Validating kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml against schema src/communitymech/schema/communitymech.yaml\nCache directory: references_cache\n\nValidation Summary:\n Total checks: 0\n All validations passed!\n" + }, + { + "check": "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-snippets", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "scripts/evidence_snippet_audit.py", + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml", + "--list-mismatch", + "--list-nocontent", + "--list-rendering", + "--list-assembled" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-snippets.log", + "log_sha256": "45007c108f9ec8bef530a5471e83cc9e84b78b05e90ad8c19de389a107ed4f60", + "output_tail": "# 7 evidence snippets scanned across 1 files\n MATCH 7\n RENDERING 0\n ASSEMBLED 0\n WEAK 0\n MISMATCH 0\n NOCONTENT 0\n\n# Files with MISMATCH/WEAK (content present but snippet absent) \u2014 fabrication suspects\n\n# MISMATCH/WEAK detail\n\n# Files by RENDERING (faithful quote; differs from cache only by\n# punctuation/whitespace/Greek \u2014 this is what validate-references flags)\n\n# Top files by NOCONTENT (unverifiable; cache stub/missing)\n\n# ASSEMBLED (every part is in the source; the join is not \u2014\n# a table flattened into prose, or a quote welded from two places)\n" + }, + { + "check": "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-rank", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "scripts/rank_causal_graph_readiness.py", + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml", + "--format", + "json" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-rank.log", + "log_sha256": "b1f6cc44261120ae405fdf2d5f9c603913e5cd408fa6346888d7ef941f27289e", + "output_tail": "[\n {\n \"rank\": 1,\n \"id\": \"CommunityMech:000343\",\n \"name\": \"Komagataella-E. coli Co-inducible Biosynthesis Coculture\",\n \"score\": 0.0,\n \"taxonomy\": 2,\n \"connected_taxa\": 0,\n \"disconnected_taxa\": 2,\n \"ecological_interactions\": 0,\n \"downstream_edges\": 0,\n \"interaction_evidence\": 0,\n \"dangling_downstream\": 0,\n \"self_downstream\": 0,\n \"missing\": [\n \"ecological_interactions\"\n ],\n \"path\": \"kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml\"\n }\n]\n" + }, + { + "check": "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-history-0", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/linkml-validate", + "-s", + "src/communitymech/schema/history.yaml", + "--target-class", + "HistoryRecord", + "history/records/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture/2026-10-06T201911Z-codex-e2d63d.yaml" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-history-0.log", + "log_sha256": "de6087f4be8c0adc015333597cd864406ad889bbde42d9ee20db0df5932bb2de", + "output_tail": "No issues found\n" + }, + { + "check": "inventory", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "reports/causal_graph_review/build_inventory.py" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/inventory.log", + "log_sha256": "02bb942a1963465c7a8112a10ebe2cc7ac34124acc7c99d230079a0c3c4413f3", + "output_tail": "{\n \"scope\": \"Every YAML under kb/communities and data/isolates; filesystem discovery includes hidden/ignored files.\",\n \"semantic_review_rule\": \"Structural checks and readiness scores do not establish causal support. Completed reviews must match the current record SHA-256, cover every node once with a rationale, and reproduce the current edge list.\",\n \"records\": 460,\n \"nodes\": 1458,\n \"edges\": 651,\n \"records_without_nodes\": 8,\n \"records_without_edges\": 156,\n \"review_status_counts\": {\n \"needs_research\": 21,\n \"pending\": 245,\n \"reviewed\": 194\n },\n \"structural_defects\": []\n}\n" + }, + { + "check": "network", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "-m", + "communitymech.cli", + "audit-network", + "--json" + ], + "exit_code": 1, + "expected_exit_codes": [ + 1 + ], + "log": "/private/tmp/communitymech-batch60/network.log", + "log_sha256": "0955d3471f474fec8dcceb846ff932573d11c9833de73b2b2822241553762186", + "output_tail": "{\n \"preferred_term\": \"Legionella MAGs\",\n \"term\": {\n \"id\": \"NCBITaxon:445\",\n \"label\": \"Legionella\"\n },\n \"gtdb_grounding_status\": \"UNRESOLVED\",\n \"notes\": \"The study detected Legionella-affiliated MAGs and used them in a virulence-gene risk assessment; the source did not assert pathogenicity for a cultured Legionella isolate.\\n\"\n },\n \"abundance_value\": \"Lower-abundance Legionella-affiliated MAGs detected during metagenomic risk assessment.\\n\",\n \"evidence\": [\n {\n \"reference\": \"PMID:41457319\",\n \"supports\": \"SUPPORT\",\n \"evidence_source\": \"COMPUTATIONAL\",\n \"snippet\": \"detection of waterborne pathogens from the Legionella genus\",\n \"explanation\": \"Supports Legionella-affiliated lower-abundance bins as monitored biosafety-relevant members of the consortium.\\n\"\n }\n ]\n },\n \"message\": \"Taxon 'Legionella MAGs' has no interactions\"\n }\n ],\n \"Sclerotinia_Sclerotia_12Strain_Biocontrol_SynCom\": [\n {\n \"type\": \"UNKNOWN_TARGET\",\n \"interaction\": \"Twelve-Strain SynCom Suppression of Sclerotinia Sclerotia\",\n \"interaction_index\": 0,\n \"taxon\": \"Sclerotinia sclerotiorum\",\n \"severity\": \"warning\",\n \"message\": \"Target taxon 'Sclerotinia sclerotiorum' not found in taxonomy section (community-level scope)\"\n },\n {\n \"type\": \"UNKNOWN_TARGET\",\n \"interaction\": \"Trinickia MC Key Contribution to Sclerotia Mortality\",\n \"interaction_index\": 1,\n \"taxon\": \"Sclerotinia sclerotiorum\",\n \"severity\": \"warning\",\n \"message\": \"Target taxon 'Sclerotinia sclerotiorum' not found in taxonomy section (community-level scope)\"\n }\n ],\n \"Space_Habitat_SevenMember_Stress_Tolerance_SynCom\": [\n {\n \"type\": \"DISCONNECTED\",\n \"taxon\": \"Acinetobacter radioresistens 50V1\",\n \"taxon_id\": \"NCBITaxon:40216\",\n \"taxon_data\": {\n \"taxon_term\": {\n \"preferred_term\": \"Acinetobacter radioresistens 50V1\",\n \"term\": {\n \"id\": \"NCBITaxon:40216\",\n \"label\": \"Acinetobacter radioresistens\"\n },\n \"gtdb_grounding_status\": \"UNRESOLVED\",\n \"notes\": \"The isolate came from the preflight Mars Odyssey Orbiter; ontology grounding is at species level.\"\n },\n \"strain_designation\": {\n \"strain_name\": \"50V1\",\n \"genome_accession\": \"GCA_002834555.1\"\n },\n \"evidence\": [\n {\n \"reference\": \"PMID:42577390\",\n \"supports\": \"SUPPORT\",\n \"evidence_source\": \"IN_VITRO\",\n \"snippet\": \"Acinetobacter radioresistens | 50V1 | Preflight Mars Odyssey Orbiter | \\u2013 | Mccoy et al., 2012 | GCA_002834555.1 | TSYB/TSYA | 37 \\u00b0C | 16\\u201318 h\",\n \"explanation\": \"Identifies A. radioresistens 50V1 as a consortium member and gives its spacecraft-associated source, genome accession and cultivation conditions.\"\n }\n ]\n },\n \"message\": \"Taxon 'Acinetobacter radioresistens 50V1' has no interactions\"\n },\n {\n \"type\": \"DISCONNECTED\",\n \"taxon\": \"Micrococcus luteus W4-Al-K1\",\n \"taxon_id\": \"NCBITaxon:1270\",\n \"taxon_data\": {\n \"taxon_term\": {\n \"preferred_term\": \"Micrococcus luteus W4-Al-K1\",\n \"term\": {\n \"id\": \"NCBITaxon:1270\",\n \"label\": \"Micrococcus luteus\"\n },\n \"gtdb_grounding_status\": \"UNRESOLVED\",\n \"notes\": \"The isolate was recovered from an ISS aluminum-case touch array; ontology grounding is at species level.\"\n },\n \"strain_designation\": {\n \"strain_name\": \"W4-Al-K1\",\n \"genome_accession\": \"GCA_057816565.1\"\n },\n \"evidence\": [\n {\n \"reference\": \"PMID:42577390\",\n \"supports\": \"SUPPORT\",\n \"evidence_source\": \"IN_VITRO\",\n \"snippet\": \"Micrococcus luteus | W4-Al-K1 | ISS, Touch Array Aluminum Case | - | Kr\\u00e4mer et al., 2025 | GCA_057816565.1 | NB, TSYB/TSYA | 37 \\u00b0C | 24 h\",\n \"explanation\": \"Identifies M. luteus W4-Al-K1 as a consortium member and gives its ISS source, genome accession and cultivation conditions.\"\n }\n ]\n },\n \"message\": \"Taxon 'Micrococcus luteus W4-Al-K1' has no interactions\"\n },\n {\n \"type\": \"DISCONNECTED\",\n \"taxon\": \"Propionibacterium cyclohexanicum TA-12T\",\n \"taxon_id\": \"NCBITaxon:64702\",\n \"taxon_data\": {\n \"taxon_term\": {\n \"preferred_term\": \"Propionibacterium cyclohexanicum TA-12T\",\n \"term\": {\n \"id\": \"NCBITaxon:64702\",\n \"label\": \"Propionibacterium cyclohexanicum\"\n },\n \"gtdb_grounding_status\": \"UNRESOLVED\",\n \"notes\": \"The source used the P. cyclohexanicum type strain as a biosafety-level-1 representative.\"\n },\n \"strain_designation\": {\n \"strain_name\": \"TA-12T\",\n \"genome_accession\": \"GCA_900111365\",\n \"culture_collections\": [\n {\n \"collection\": \"DSM\",\n \"accession\": \"16859\"\n }\n ]\n },\n \"evidence\": [\n {\n \"reference\": \"PMID:42577390\",\n \"supports\": \"SUPPORT\",\n \"evidence_source\": \"IN_VITRO\",\n \"snippet\": \"Propionibacterium cyclohexanicum | TA-12 T | Spoilt Orange Juice | DSM 16859 T | Kusano et al., 1997 | GCA_900111365 | BHI, TSYB/TSYA | 37 \\u00b0C | 24 h\",\n \"explanation\": \"Identifies P. cyclohexanicum TA-12T as a consortium member and gives its source, DSM number, genome accession and cultivation conditions.\"\n }\n ]\n },\n \"message\": \"Taxon 'Propionibacterium cyclohexanicum TA-12T' has no interactions\"\n },\n {\n \"type\": \"DISCONNECTED\",\n \"taxon\": \"Pseudomonas antarctica CMS 35T\",\n \"taxon_id\": \"NCBITaxon:219572\",\n \"taxon_data\": {\n \"taxon_term\": {\n \"preferred_term\": \"Pseudomonas antarctica CMS 35T\",\n \"term\": {\n \"id\": \"NCBITaxon:219572\",\n \"label\": \"Pseudomonas antarctica\"\n },\n \"gtdb_grounding_status\": \"UNRESOLVED\",\n \"notes\": \"The source used the P. antarctica type strain as a biosafety-level-1 Pseudomonas surrogate.\"\n },\n \"strain_designation\": {\n \"strain_name\": \"CMS 35T\",\n \"genome_accession\": \"GCA_010634845\",\n \"culture_collections\": [\n {\n \"collection\": \"DSM\",\n \"accession\": \"15318\"\n }\n ]\n },\n \"evidence\": [\n {\n \"reference\": \"PMID:42577390\",\n \"supports\": \"SUPPORT\",\n \"evidence_source\": \"IN_VITRO\",\n \"snippet\": \"Pseudomonas antarctica | CMS 35 T | Antarctica | DSM 15318 T | Reddy et al., 2004 | GCA_010634845 | TSYB/TSYA | Ambient | 12\\u201316 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\"supports\": \"SUPPORT\",\n \"evidence_source\": \"IN_VITRO\",\n \"snippet\": \"Staphylococcus capitis | K1-2-2-23 | ISS, V2A Steel | DSM 111179 | Sobisch et al., 2019 | GCA_025272695 | TSYB/TSYA | 37 \\u00b0C | 16\\u201324 h\",\n \"explanation\": \"Identifies S. capitis K1-2-2-23 as a consortium member and gives its source, DSM number, genome accession and cultivation conditions.\"\n }\n ]\n },\n \"message\": \"Taxon 'Staphylococcus capitis K1-2-2-23' has no interactions\"\n },\n {\n \"type\": \"DISCONNECTED\",\n \"taxon\": \"Streptococcus halotolerans HTS9T\",\n \"taxon_id\": \"NCBITaxon:1814128\",\n \"taxon_data\": {\n \"taxon_term\": {\n \"preferred_term\": \"Streptococcus halotolerans HTS9T\",\n \"term\": {\n \"id\": \"NCBITaxon:1814128\",\n \"label\": \"Streptococcus halotolerans\"\n },\n \"gtdb_grounding_status\": \"UNRESOLVED\",\n \"notes\": \"The source used the S. halotolerans type strain as a biosafety-level-1 representative.\"\n },\n \"strain_designation\": {\n 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Community\t6\t6\t0\t5\t3\t6\t\t0\t0\n441\t100.0\tkb/communities/Tobacco_Chemotactic_Biocontrol_SynCom.yaml\tCommunityMech:000106\tTobacco Chemotactic Biocontrol SynCom\t2\t2\t0\t3\t2\t4\t\t0\t0\n442\t100.0\tkb/communities/Tomato_Oxylipin_SynCom3.yaml\tCommunityMech:000107\tTomato Oxylipin-Protective SynCom3\t3\t3\t0\t3\t2\t4\t\t0\t0\n443\t100.0\tkb/communities/Tribromophenol_Anaerobic_Bioremediation_SynCom.yaml\tCommunityMech:000112\tTribromophenol Anaerobic Bioremediation SynCom\t3\t3\t0\t4\t3\t5\t\t0\t0\n444\t100.0\tkb/communities/Trichococcus_Syntrophomonas_Methanospirillum_Butyrate_Coculture.yaml\tCommunityMech:000188\tTrichococcus-Syntrophomonas-Methanospirillum Butyrate Coculture\t3\t3\t0\t3\t2\t6\t\t0\t0\n445\t100.0\tkb/communities/Trichoderma_Ecoli_Cellulosic_Isobutanol_Coculture.yaml\tCommunityMech:000205\tTrichoderma-E. coli Cellulosic Isobutanol Coculture\t2\t2\t0\t3\t2\t5\t\t0\t0\n446\t100.0\tkb/communities/Trichoderma_Lactate_Platform.yaml\tCommunityMech:000075\tTrichoderma Lactate Platform for SCFA Production\t3\t3\t0\t3\t2\t3\t\t0\t0\n447\t100.0\tkb/communities/Trichoderma_Streptomyces_Filamentous_Cellulose_Coculture.yaml\tCommunityMech:000270\tTrichoderma-Streptomyces Filamentous Cellulose Coculture\t2\t2\t0\t3\t2\t7\t\t0\t0\n448\t100.0\tkb/communities/Trichodesmium_Alteromonas_Marine_Consortium.yaml\tCommunityMech:000147\tTrichodesmium-Alteromonas Marine Consortium\t3\t3\t0\t3\t2\t5\t\t0\t0\n449\t100.0\tkb/communities/Tropidoatractus_Magnetotacticus_Tripartite_Syntrophy.yaml\tCommunityMech:000337\tTropidoatractus magnetotacticus Magnetotactic Ciliate Tripartite Syntrophy\t3\t3\t0\t4\t2\t9\t\t0\t0\n450\t100.0\tkb/communities/Urine_Nitrification_SynCom.yaml\tCommunityMech:000117\tUrine Nitrification Synthetic Microbial Community\t5\t5\t0\t3\t2\t5\t\t0\t0\n451\t100.0\tkb/communities/Variovorax_Cryptococcus_Vitamin_Mutualism_Microcosm.yaml\tCommunityMech:000213\tVariovorax-Cryptococcus Vitamin Cross-Feeding Microcosm\t2\t2\t0\t3\t2\t4\t\t0\t0\n452\t100.0\tkb/communities/Waste_Sludge_Electrofermentation_Biofilm_Suspension_Community.yaml\tCommunityMech:000328\tWaste-Sludge Electro-Fermentation Biofilm-Suspension Community\t5\t5\t0\t4\t2\t6\t\t0\t0\n453\t100.0\tkb/communities/Watermelon_Rhizosphere_Fusarium_SynCom8.yaml\tCommunityMech:000098\tWatermelon Rhizosphere Fusarium-Protective SynCom8\t2\t2\t0\t3\t2\t7\t\t0\t0\n454\t100.0\tkb/communities/Wetland_Oxygen_Sulfate_GHG_Microcosm_Community.yaml\tCommunityMech:000255\tWetland Oxygen-Sulfate Greenhouse Gas Microcosm Community\t3\t3\t0\t3\t2\t6\t\t0\t0\n455\t100.0\tkb/communities/Wheat_Straw_Biogas_Pretreatment_SynCom.yaml\tCommunityMech:000118\tWheat Straw Biogas Pretreatment SynCom\t4\t4\t0\t3\t2\t4\t\t0\t0\n456\t100.0\tkb/communities/Yarrowia_lipolytica_Division_of_Labor_Lipid_Consortium.yaml\tCommunityMech:000354\tYarrowia lipolytica Division-of-Labor Lipid Consortium\t2\t2\t0\t3\t2\t3\t\t0\t0\n457\t100.0\tkb/communities/Yogurt_TwoSpecies_Starter_Culture.yaml\tCommunityMech:000164\tYogurt Two-Species Starter Culture\t2\t2\t0\t4\t2\t9\t\t0\t0\n458\t100.0\tkb/communities/Zymomonas_Ecoli_Exometabolomics_Obligate_Mutualism.yaml\tCommunityMech:000210\tZymomonas-E. coli Exometabolomics-Designed Obligate Mutualism\t2\t2\t0\t3\t2\t6\t\t0\t0\n459\t100.0\tkb/communities/hCom2_Complex_Gut_Microbiome.yaml\tCommunityMech:000123\thCom2 Complex Gut Microbiome\t1\t1\t0\t3\t2\t3\t\t0\t0\n460\t100.0\tkb/communities/mCAFEs_Brachypodium_RCC.yaml\tCommunityMech:000078\tm-CAFEs Brachypodium Reduced Complexity Consortia\t10\t10\t0\t6\t2\t11\t\t0\t0\nPYTHONPATH=src uv run python scripts/rank_causal_graph_readiness.py \n" + }, + { + "check": "render", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "-m", + "communitymech.render" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/render.log", + "log_sha256": "6be6dfeb9e47171b7569855d3facb0c059b19dbd8f364589f82e0d8a623d46f2", + "output_tail": "sm.html\n \u2713 Synechococcus_Bacillus_SPC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Bacillus_SPC.html\n \u2713 Synechococcus_Ecoli_SPC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Ecoli_SPC.html\n \u2713 Synechococcus_Halomonas_Light_Driven_PHB_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Halomonas_Light_Driven_PHB_Coculture.html\n \u2713 Synechococcus_Pseudomonas_PhotoPHA_DNT_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Pseudomonas_PhotoPHA_DNT_Coculture.html\n \u2713 Synechococcus_Saccharomyces_SPC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Saccharomyces_SPC.html\n \u2713 Synechococcus_Shewanella_Dlactate_Biophotovoltaic_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Shewanella_Dlactate_Biophotovoltaic_Consortium.html\n \u2713 Synechocystis_Pseudomonas_Acetate_Butanol_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechocystis_Pseudomonas_Acetate_Butanol_Coculture.html\n \u2713 Synthetic_Lichen_Synechococcus_Rhodotorula_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synthetic_Lichen_Synechococcus_Rhodotorula_Coculture.html\n \u2713 Synthetic_Periphyton_Freshwater_Biofilm.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synthetic_Periphyton_Freshwater_Biofilm.html\n \u2713 Syntrophobacter_Methanobacterium_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophobacter_Methanobacterium_Syntrophy.html\n \u2713 Syntrophobacter_Methanospirillum_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophobacter_Methanospirillum_Syntrophy.html\n \u2713 Syntrophomonas_Methanococcus_Butyrate_Growth_Coordination_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophomonas_Methanococcus_Butyrate_Growth_Coordination_Coculture.html\n \u2713 Syntrophomonas_Methanospirillum_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophomonas_Methanospirillum_Syntrophy.html\n \u2713 Syntrophus_Benzoate_Degrader.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophus_Benzoate_Degrader.html\n \u2713 Syntrophus_Methanospirillum_Gentianae_Benzoate_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophus_Methanospirillum_Gentianae_Benzoate_Coculture.html\n \u2713 TCP_NonDehalogenimonas_Groundwater_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/TCP_NonDehalogenimonas_Groundwater_Consortium.html\n \u2713 THOR_Rhizosphere_Model_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/THOR_Rhizosphere_Model_Community.html\n \u2713 TYQ1_Nematode_Biocontrol_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/TYQ1_Nematode_Biocontrol_SynCom.html\n \u2713 Teosinte_Maize_Biofertilizer_SynCom7.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Teosinte_Maize_Biofertilizer_SynCom7.html\n \u2713 Thalassiosira_Marinobacter_Marine_Snow_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thalassiosira_Marinobacter_Marine_Snow_Coculture.html\n \u2713 Thalassiosira_Ruegeria_Phycosphere_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thalassiosira_Ruegeria_Phycosphere_Coculture.html\n \u2713 Thermacetogenium_Methanothermobacter_Acetate_Oxidation_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermacetogenium_Methanothermobacter_Acetate_Oxidation_Coculture.html\n \u2713 Thermoleptolyngbya_Chelatococcus_Arsenic_Detoxification_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermoleptolyngbya_Chelatococcus_Arsenic_Detoxification_Coculture.html\n \u2713 Thermophilic_ExSitu_Biomethanation_Mixed_Culture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermophilic_ExSitu_Biomethanation_Mixed_Culture.html\n \u2713 Thermophilic_Lignocellulose_Composting_SynCom_Biosanitization.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermophilic_Lignocellulose_Composting_SynCom_Biosanitization.html\n \u2713 Thermophilic_Pyrite_QS_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermophilic_Pyrite_QS_Consortium.html\n \u2713 Thermotoga_Methanocaldococcus_Hyperthermophilic_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermotoga_Methanocaldococcus_Hyperthermophilic_Syntrophy.html\n \u2713 Thiocyanate_Afipia_Thiobacillus_Bioreactor_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thiocyanate_Afipia_Thiobacillus_Bioreactor_Community.html\n \u2713 Tinto_River_Iron_Cycling_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tinto_River_Iron_Cycling_Community.html\n \u2713 Tobacco_Chemotactic_Biocontrol_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tobacco_Chemotactic_Biocontrol_SynCom.html\n \u2713 Tomato_Oxylipin_SynCom3.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tomato_Oxylipin_SynCom3.html\n \u2713 Tribromophenol_Anaerobic_Bioremediation_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tribromophenol_Anaerobic_Bioremediation_SynCom.html\n \u2713 Trichococcus_Syntrophomonas_Methanospirillum_Butyrate_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichococcus_Syntrophomonas_Methanospirillum_Butyrate_Coculture.html\n \u2713 Trichoderma_Ecoli_Cellulosic_Isobutanol_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichoderma_Ecoli_Cellulosic_Isobutanol_Coculture.html\n \u2713 Trichoderma_Lactate_Platform.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichoderma_Lactate_Platform.html\n \u2713 Trichoderma_Streptomyces_Filamentous_Cellulose_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichoderma_Streptomyces_Filamentous_Cellulose_Coculture.html\n \u2713 Trichodesmium_Alteromonas_Marine_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichodesmium_Alteromonas_Marine_Consortium.html\n \u2713 Tropidoatractus_Magnetotacticus_Tripartite_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tropidoatractus_Magnetotacticus_Tripartite_Syntrophy.html\n \u2713 Urine_Nitrification_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Urine_Nitrification_SynCom.html\n \u2713 Variovorax_Cryptococcus_Vitamin_Mutualism_Microcosm.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Variovorax_Cryptococcus_Vitamin_Mutualism_Microcosm.html\n \u2713 Viili_Fungus_Bacterium_EPS_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Viili_Fungus_Bacterium_EPS_SynCom.html\n \u2713 Waste_Sludge_Electrofermentation_Biofilm_Suspension_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Waste_Sludge_Electrofermentation_Biofilm_Suspension_Community.html\n \u2713 Watermelon_Rhizosphere_Fusarium_SynCom8.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Watermelon_Rhizosphere_Fusarium_SynCom8.html\n \u2713 Wetland_Oxygen_Sulfate_GHG_Microcosm_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wetland_Oxygen_Sulfate_GHG_Microcosm_Community.html\n \u2713 Wheat_Consortium_C1.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_Consortium_C1.html\n \u2713 Wheat_Consortium_C6.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_Consortium_C6.html\n \u2713 Wheat_CrownRot_SixStrain_CrossKingdom_SMC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_CrownRot_SixStrain_CrossKingdom_SMC.html\n \u2713 Wheat_CrownRot_TB_CrossKingdom_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_CrownRot_TB_CrossKingdom_SynCom.html\n \u2713 Wheat_DT_Rhizosphere_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_DT_Rhizosphere_SynCom.html\n \u2713 Wheat_Straw_Biogas_Pretreatment_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_Straw_Biogas_Pretreatment_SynCom.html\n \u2713 Wheat_SynCom_SASW01_Drought_Inoculant.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_SynCom_SASW01_Drought_Inoculant.html\n \u2713 Wine_Yeast_FiveSpecies_Strain_Substitution_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wine_Yeast_FiveSpecies_Strain_Substitution_Consortium.html\n \u2713 Wine_Yeast_ThreeSpecies_Contact_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wine_Yeast_ThreeSpecies_Contact_Consortium.html\n \u2713 Wolffia_Mankai_Endosphere_Cobamide_Guild.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wolffia_Mankai_Endosphere_Cobamide_Guild.html\n \u2713 Wollastonite_Ramichloridium_Talaromyces_Fungal_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wollastonite_Ramichloridium_Talaromyces_Fungal_Consortium.html\n \u2713 Yarrowia_lipolytica_Division_of_Labor_Lipid_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Yarrowia_lipolytica_Division_of_Labor_Lipid_Consortium.html\n \u2713 Yogurt_TwoSpecies_Starter_Culture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Yogurt_TwoSpecies_Starter_Culture.html\n \u2713 Zymomonas_Ecoli_Exometabolomics_Obligate_Mutualism.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Zymomonas_Ecoli_Exometabolomics_Obligate_Mutualism.html\n \u2713 hCom2_Complex_Gut_Microbiome.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/hCom2_Complex_Gut_Microbiome.html\n \u2713 mCAFEs_Brachypodium_RCC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/mCAFEs_Brachypodium_RCC.html\n\n\u2705 Rendered 456 communities to /private/tmp/communitymech-causal-batch60/docs/communities\n \u2713 Generated browser at /private/tmp/communitymech-causal-batch60/docs/browser.html\n \u2713 Generated landing at /private/tmp/communitymech-causal-batch60/docs/index.html\n" + }, + { + "check": "render-repeat", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "-m", + "communitymech.render" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/render-repeat.log", + "log_sha256": "6be6dfeb9e47171b7569855d3facb0c059b19dbd8f364589f82e0d8a623d46f2", + "output_tail": "sm.html\n \u2713 Synechococcus_Bacillus_SPC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Bacillus_SPC.html\n \u2713 Synechococcus_Ecoli_SPC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Ecoli_SPC.html\n \u2713 Synechococcus_Halomonas_Light_Driven_PHB_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Halomonas_Light_Driven_PHB_Coculture.html\n \u2713 Synechococcus_Pseudomonas_PhotoPHA_DNT_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Pseudomonas_PhotoPHA_DNT_Coculture.html\n \u2713 Synechococcus_Saccharomyces_SPC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Saccharomyces_SPC.html\n \u2713 Synechococcus_Shewanella_Dlactate_Biophotovoltaic_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechococcus_Shewanella_Dlactate_Biophotovoltaic_Consortium.html\n \u2713 Synechocystis_Pseudomonas_Acetate_Butanol_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synechocystis_Pseudomonas_Acetate_Butanol_Coculture.html\n \u2713 Synthetic_Lichen_Synechococcus_Rhodotorula_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synthetic_Lichen_Synechococcus_Rhodotorula_Coculture.html\n \u2713 Synthetic_Periphyton_Freshwater_Biofilm.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Synthetic_Periphyton_Freshwater_Biofilm.html\n \u2713 Syntrophobacter_Methanobacterium_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophobacter_Methanobacterium_Syntrophy.html\n \u2713 Syntrophobacter_Methanospirillum_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophobacter_Methanospirillum_Syntrophy.html\n \u2713 Syntrophomonas_Methanococcus_Butyrate_Growth_Coordination_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophomonas_Methanococcus_Butyrate_Growth_Coordination_Coculture.html\n \u2713 Syntrophomonas_Methanospirillum_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophomonas_Methanospirillum_Syntrophy.html\n \u2713 Syntrophus_Benzoate_Degrader.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophus_Benzoate_Degrader.html\n \u2713 Syntrophus_Methanospirillum_Gentianae_Benzoate_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Syntrophus_Methanospirillum_Gentianae_Benzoate_Coculture.html\n \u2713 TCP_NonDehalogenimonas_Groundwater_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/TCP_NonDehalogenimonas_Groundwater_Consortium.html\n \u2713 THOR_Rhizosphere_Model_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/THOR_Rhizosphere_Model_Community.html\n \u2713 TYQ1_Nematode_Biocontrol_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/TYQ1_Nematode_Biocontrol_SynCom.html\n \u2713 Teosinte_Maize_Biofertilizer_SynCom7.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Teosinte_Maize_Biofertilizer_SynCom7.html\n \u2713 Thalassiosira_Marinobacter_Marine_Snow_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thalassiosira_Marinobacter_Marine_Snow_Coculture.html\n \u2713 Thalassiosira_Ruegeria_Phycosphere_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thalassiosira_Ruegeria_Phycosphere_Coculture.html\n \u2713 Thermacetogenium_Methanothermobacter_Acetate_Oxidation_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermacetogenium_Methanothermobacter_Acetate_Oxidation_Coculture.html\n \u2713 Thermoleptolyngbya_Chelatococcus_Arsenic_Detoxification_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermoleptolyngbya_Chelatococcus_Arsenic_Detoxification_Coculture.html\n \u2713 Thermophilic_ExSitu_Biomethanation_Mixed_Culture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermophilic_ExSitu_Biomethanation_Mixed_Culture.html\n \u2713 Thermophilic_Lignocellulose_Composting_SynCom_Biosanitization.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermophilic_Lignocellulose_Composting_SynCom_Biosanitization.html\n \u2713 Thermophilic_Pyrite_QS_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermophilic_Pyrite_QS_Consortium.html\n \u2713 Thermotoga_Methanocaldococcus_Hyperthermophilic_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thermotoga_Methanocaldococcus_Hyperthermophilic_Syntrophy.html\n \u2713 Thiocyanate_Afipia_Thiobacillus_Bioreactor_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Thiocyanate_Afipia_Thiobacillus_Bioreactor_Community.html\n \u2713 Tinto_River_Iron_Cycling_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tinto_River_Iron_Cycling_Community.html\n \u2713 Tobacco_Chemotactic_Biocontrol_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tobacco_Chemotactic_Biocontrol_SynCom.html\n \u2713 Tomato_Oxylipin_SynCom3.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tomato_Oxylipin_SynCom3.html\n \u2713 Tribromophenol_Anaerobic_Bioremediation_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tribromophenol_Anaerobic_Bioremediation_SynCom.html\n \u2713 Trichococcus_Syntrophomonas_Methanospirillum_Butyrate_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichococcus_Syntrophomonas_Methanospirillum_Butyrate_Coculture.html\n \u2713 Trichoderma_Ecoli_Cellulosic_Isobutanol_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichoderma_Ecoli_Cellulosic_Isobutanol_Coculture.html\n \u2713 Trichoderma_Lactate_Platform.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichoderma_Lactate_Platform.html\n \u2713 Trichoderma_Streptomyces_Filamentous_Cellulose_Coculture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichoderma_Streptomyces_Filamentous_Cellulose_Coculture.html\n \u2713 Trichodesmium_Alteromonas_Marine_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Trichodesmium_Alteromonas_Marine_Consortium.html\n \u2713 Tropidoatractus_Magnetotacticus_Tripartite_Syntrophy.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Tropidoatractus_Magnetotacticus_Tripartite_Syntrophy.html\n \u2713 Urine_Nitrification_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Urine_Nitrification_SynCom.html\n \u2713 Variovorax_Cryptococcus_Vitamin_Mutualism_Microcosm.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Variovorax_Cryptococcus_Vitamin_Mutualism_Microcosm.html\n \u2713 Viili_Fungus_Bacterium_EPS_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Viili_Fungus_Bacterium_EPS_SynCom.html\n \u2713 Waste_Sludge_Electrofermentation_Biofilm_Suspension_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Waste_Sludge_Electrofermentation_Biofilm_Suspension_Community.html\n \u2713 Watermelon_Rhizosphere_Fusarium_SynCom8.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Watermelon_Rhizosphere_Fusarium_SynCom8.html\n \u2713 Wetland_Oxygen_Sulfate_GHG_Microcosm_Community.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wetland_Oxygen_Sulfate_GHG_Microcosm_Community.html\n \u2713 Wheat_Consortium_C1.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_Consortium_C1.html\n \u2713 Wheat_Consortium_C6.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_Consortium_C6.html\n \u2713 Wheat_CrownRot_SixStrain_CrossKingdom_SMC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_CrownRot_SixStrain_CrossKingdom_SMC.html\n \u2713 Wheat_CrownRot_TB_CrossKingdom_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_CrownRot_TB_CrossKingdom_SynCom.html\n \u2713 Wheat_DT_Rhizosphere_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_DT_Rhizosphere_SynCom.html\n \u2713 Wheat_Straw_Biogas_Pretreatment_SynCom.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_Straw_Biogas_Pretreatment_SynCom.html\n \u2713 Wheat_SynCom_SASW01_Drought_Inoculant.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wheat_SynCom_SASW01_Drought_Inoculant.html\n \u2713 Wine_Yeast_FiveSpecies_Strain_Substitution_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wine_Yeast_FiveSpecies_Strain_Substitution_Consortium.html\n \u2713 Wine_Yeast_ThreeSpecies_Contact_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wine_Yeast_ThreeSpecies_Contact_Consortium.html\n \u2713 Wolffia_Mankai_Endosphere_Cobamide_Guild.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wolffia_Mankai_Endosphere_Cobamide_Guild.html\n \u2713 Wollastonite_Ramichloridium_Talaromyces_Fungal_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Wollastonite_Ramichloridium_Talaromyces_Fungal_Consortium.html\n \u2713 Yarrowia_lipolytica_Division_of_Labor_Lipid_Consortium.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Yarrowia_lipolytica_Division_of_Labor_Lipid_Consortium.html\n \u2713 Yogurt_TwoSpecies_Starter_Culture.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Yogurt_TwoSpecies_Starter_Culture.html\n \u2713 Zymomonas_Ecoli_Exometabolomics_Obligate_Mutualism.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/Zymomonas_Ecoli_Exometabolomics_Obligate_Mutualism.html\n \u2713 hCom2_Complex_Gut_Microbiome.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/hCom2_Complex_Gut_Microbiome.html\n \u2713 mCAFEs_Brachypodium_RCC.yaml \u2192 /private/tmp/communitymech-causal-batch60/docs/communities/mCAFEs_Brachypodium_RCC.html\n\n\u2705 Rendered 456 communities to /private/tmp/communitymech-causal-batch60/docs/communities\n \u2713 Generated browser at /private/tmp/communitymech-causal-batch60/docs/browser.html\n \u2713 Generated landing at /private/tmp/communitymech-causal-batch60/docs/index.html\n" + }, + { + "check": "connectivity-survey", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "-c", + "import json, pathlib, runpy\nfn=runpy.run_path('tests/test_community_level_connectivity_credit.py')['_survey']\nresult={}\nfor name,root in [('baseline',pathlib.Path('/private/tmp/communitymech-causal-batch59')),('current',pathlib.Path.cwd())]:\n paths=sorted(p for r in ['kb/communities','data/isolates'] for p in (root/r).rglob('*.yaml'))\n assert len(paths)==460\n fn.__globals__['record_files']=lambda: paths\n result[name]=fn()\nprint(json.dumps(result))\n" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/connectivity-survey.log", + "log_sha256": "669dbb91e596e26f351635bde2c0e185fe30e7f801d0c0ee0c808e9c11f0ca8e", + "output_tail": "{\"baseline\": {\"records\": 460, \"with_community_level\": 390, \"mixed\": 124, \"community_level_only\": 266, \"taxa\": 1505, \"credited_solely_by_the_rule\": 1236}, \"current\": {\"records\": 460, \"with_community_level\": 391, \"mixed\": 124, \"community_level_only\": 267, \"taxa\": 1507, \"credited_solely_by_the_rule\": 1238}}\n" + }, + { + "check": "regressions", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "-m", + "pytest", + "-q", + "tests/test_causal_review_batch10.py", + "tests/test_causal_review_batch11.py", + "tests/test_causal_review_batch12.py", + "tests/test_causal_review_batch13.py", + "tests/test_causal_review_batch14.py", + "tests/test_causal_review_batch15.py", + "tests/test_causal_review_batch16.py", + "tests/test_causal_review_batch17.py", + "tests/test_causal_review_batch18.py", + "tests/test_causal_review_batch19.py", + "tests/test_causal_review_batch20.py", + "tests/test_causal_review_batch21.py", + "tests/test_causal_review_batch22.py", + "tests/test_causal_review_batch23.py", + "tests/test_causal_review_batch24.py", + "tests/test_causal_review_batch25.py", + "tests/test_causal_review_batch27.py", + "tests/test_causal_review_batch28.py", + "tests/test_causal_review_batch29.py", + "tests/test_causal_review_batch30.py", + "tests/test_causal_review_batch31.py", + "tests/test_causal_review_batch32.py", + "tests/test_causal_review_batch33.py", + "tests/test_causal_review_batch34.py", + "tests/test_causal_review_batch35.py", + "tests/test_causal_review_batch36.py", + "tests/test_causal_review_batch37.py", + "tests/test_causal_review_batch38.py", + "tests/test_causal_review_batch39.py", + "tests/test_causal_review_batch40.py", + "tests/test_causal_review_batch41.py", + "tests/test_causal_review_batch42.py", + "tests/test_causal_review_batch43.py", + "tests/test_causal_review_batch44.py", + "tests/test_causal_review_batch45.py", + "tests/test_causal_review_batch46.py", + "tests/test_causal_review_batch47.py", + "tests/test_causal_review_batch48.py", + "tests/test_causal_review_batch49.py", + "tests/test_causal_review_batch5.py", + "tests/test_causal_review_batch50.py", + "tests/test_causal_review_batch51.py", + "tests/test_causal_review_batch52.py", + "tests/test_causal_review_batch53.py", + "tests/test_causal_review_batch54.py", + "tests/test_causal_review_batch55.py", + "tests/test_causal_review_batch56.py", + "tests/test_causal_review_batch57.py", + "tests/test_causal_review_batch58.py", + "tests/test_causal_review_batch59.py", + "tests/test_causal_review_batch6.py", + "tests/test_causal_review_batch60.py", + "tests/test_causal_review_batch7.py", + "tests/test_causal_review_batch8.py", + "tests/test_causal_review_batch9.py", + "tests/test_site_audit_fixes.py", + "tests/test_causal_review_inventory.py", + "tests/test_rank_causal_graph_readiness.py", + "tests/test_network_auditor.py", + "tests/test_participating_taxa.py", + "tests/test_causal_descriptions_are_published.py", + "tests/test_published_pages_name_real_records.py", + "tests/test_reference_validator_actually_validates.py", + "tests/test_snippet_audit_artefact_classes.py", + "tests/test_reference_error_annotation.py", + "tests/test_community_level_connectivity_credit.py", + "tests/test_no_paywalled_text_in_repo.py" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/regressions.log", + "log_sha256": "24411ddef190b7952d5bf89f134f4c5e573e439b5153a5382666d0b32a115f00", + "output_tail": "........................................................................ [ 13%]\n........................................................................ [ 26%]\n........................................................................ [ 39%]\n........................................................................ [ 52%]\n........................................................................ [ 65%]\n........................................................................ [ 78%]\n........................................................................ [ 91%]\n................................................ [100%]\n552 passed in 188.20s (0:03:08)\n" + }, + { + "check": "black", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/3Q_kifQtLB624D5bBWGIf/bin/black", + "--check", + "tests/test_causal_review_batch60.py", + "tests/test_participating_taxa.py" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/black.log", + "log_sha256": "b9cf511501d306d2bd8355ccb165c8ddc0a6fdd62d7c72c4a7b1795cf8934c8c", + "output_tail": "All done! \u2728 \ud83c\udf70 \u2728\n2 files would be left unchanged.\n" + }, + { + "check": "ruff", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/wCcxEYRM4HeHgwYPZemHX/bin/ruff", + "check", + "tests/test_causal_review_batch60.py", + "tests/test_participating_taxa.py" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/ruff.log", + "log_sha256": "82b3e6a6c090a57601d22943bd23fca9218d1031dbe5a7b754092f9a156b4f18", + "output_tail": "All checks passed!\n" + }, + { + "check": "diff-check", + "command": [ + "git", + "diff", + "--check" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/diff-check.log", + "log_sha256": "e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855", + "output_tail": "" + }, + { + "check": "primary-and-dryrun-proof", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "-c", + "import hashlib,json,pathlib,xml.etree.ElementTree as ET,yaml\nledger=yaml.safe_load(pathlib.Path('reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml').read_text())\nfor path,digest in ledger['primary_artifacts'].items():\n assert hashlib.sha256(pathlib.Path(path).read_bytes()).hexdigest()==digest\nfragment=ledger['primary_matched_fragment']\nprimary=pathlib.Path('/private/tmp/communitymech-batch60/kombucha-primary.txt').read_text()\nassert ''.join(fragment.split()) in ''.join(primary.split())\nassert ''.join(fragment.split()) in ''.join(pathlib.Path('references_cache/PMID_26061774.md').read_text().split())\nroot=ET.parse('/private/tmp/communitymech-batch60/kz-correction-primary.xml').getroot()\nassert root.find('.//{*}openaccessArticle').text=='false'\nassert [child.tag.split('}')[-1] for child in root]==['coredata']\nassert root.find('.//{*}originalText') is None\nd=ledger['edison_dry_run']; m=yaml.safe_load(pathlib.Path(d['path']).read_text())\nassert m['status']=='dry-run' and len(m['query'])==7457\nassert hashlib.sha256(m['query'].encode()).hexdigest()==d['query_sha256']\nassert d['provider_submissions']==d['credits_spent']==0\nprint(json.dumps({'kmc_primary_matched_arrow_fragment':True,'kz_correction_response':'core metadata only; body unread','komagataella_paid_submissions':0,'graph_extension_complete':False}))\n" + ], + "exit_code": 0, + "expected_exit_codes": [ + 0 + ], + "log": "/private/tmp/communitymech-batch60/primary-and-dryrun-proof.log", + "log_sha256": "cc47416a0f09841e9e9d73c6f6bb86ac3d47e00f913f0439fa83b6d6bb3ebbb0", + "output_tail": "{\"kmc_primary_matched_arrow_fragment\": true, \"kz_correction_response\": \"core metadata only; body unread\", \"komagataella_paid_submissions\": 0, \"graph_extension_complete\": false}\n" + } + ], + "limitations": [ + "All four original nodes and two arrows assessed; one explicit source model retained as HYPOTHESIZED and one unsupported endpoint removed. No new causal structure.", + "KZ positive performance and assay-inferred roles preserved. Primary full Methods/supplements and correction body unavailable; needs_research under1541. Publisher API metadata contradicts third-party OA labels; no access-control bypass.", + "KMC full primary main text and main/supplement captions read. Numerical Table 1 cells, original figures, supplementary PDF and raw data not inspected. Cooperation is a source model, not demonstrated reciprocal mediation.", + "Komagataella abstract and public publisher Introduction/section previews read, not full Methods/supplements. Empty graph remains needs_research under1542; no new nodes/arrows. One reviewed 7457-character Edison dry run, zero provider submissions or credits.", + "Canonical taxa and raw non-graph blocks preserved, not recertified. KMC metadata1543 and research1541/1542 remain OPEN after graph repair merge.", + "All920 reference caches unchanged. Primary-matched graph fragment does not certify full historical cache provenance or SupportingReference coverage476; Linux DOI lookup1091 and writer artifact1095 remain limitations.", + "Deduplication searched839 all-state issue titles/bodies and19 scoped ignored/hidden local matches; all three matching issue bodies read, not every comment/PR or local match.", + "Self-adversarial review is COMMENT, not independent approval. Repair1539/1540 remain OPEN until protected-main merge.", + "HTML descriptions checked by exact parser and repeat renders, not browser screenshots. Post-commit docs-current is a separate publication gate." + ], + "snippet_counts_by_record": { + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml": { + "MATCH": 13, + "RENDERING": 0, + "ASSEMBLED": 0, + "WEAK": 0, + "MISMATCH": 0, + "NOCONTENT": 0 + }, + "kb/communities/Kombucha_KMC_IMBG1_Fermentation_Community.yaml": { + "MATCH": 22, + "RENDERING": 0, + "ASSEMBLED": 0, + "WEAK": 0, + "MISMATCH": 0, + "NOCONTENT": 0 + }, + "kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml": { + "MATCH": 7, + "RENDERING": 0, + "ASSEMBLED": 0, + "WEAK": 0, + "MISMATCH": 0, + "NOCONTENT": 0 + } + }, + "unchanged_raw_blocks": 46, + "unchanged_record_files": 457, + "unchanged_cache_files": 920, + "edison_dry_run": { + "path": "research/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-edison-literature-causal-meta.yaml", + "sha256": "753bcec2844b7256e2790a9907e2c2e2d0d18d3a01fe3d39dbd9d79db395b328", + "query_sha256": "e429f0f613ac80c47e2e8ddfd83510abb8f87ba13c495f0d19a087207a5c33bb", + "query_chars": 7457, + "job": "LITERATURE", + "status": "dry-run", + "provider_submissions": 0, + "credits_spent": 0, + "paid_authorization": "specific one-job request pending" + }, + "primary_artifacts": { + "/private/tmp/communitymech-batch60/kombucha-primary.html": "d9510aedb4e415469d8568c4474b0f64f8f4dac15c8f58ef41336be82ee3be56", + "/private/tmp/communitymech-batch60/kombucha-primary.txt": "7fd7c46845afdfea0743fd39b6f10ee06f8455153f81f70d361d96d242704315", + "/private/tmp/communitymech-batch60/kz-indexed-primary.txt": "ee5dc9e5e44dd3d9f49d340b103f8552f9649d1473832c34791f53409abb3b35", + "/private/tmp/communitymech-batch60/kz-correction-crossref.json": "b76e5ed2cca7b4fe4e2b437ef281802c132589686d470f38b9f6ca05696e555b", + "/private/tmp/communitymech-batch60/kz-correction-primary.xml": "96f5611f4df555213ab98248c778172ada028c941e5cdee3b067000cdb4886ec", + "/private/tmp/communitymech-batch60/komagataella-indexed-primary.txt": "76a968c26f974b084b3b61102f6dcda072a308615999e3144d2cc66aad8c517b" + }, + "cache_provenance_caveat": "All 920 caches unchanged. The retained KMC arrow fragment independently matches fresh publisher primary text; this does not certify full historical cache provenance or scientific support for non-graph fields.", + "network_delta": { + "existing_findings_preserved": 36, + "added_findings": {}, + "removed_findings": {} + }, + "render_determinism": true, + "published_description_coverage": { + "pages": 456, + "nodes": 1444, + "arrows": 643 + }, + "participant_census": { + "measurements": { + "baseline": { + "records": 460, + "with_community_level": 390, + "mixed": 124, + "community_level_only": 266, + "taxa": 1505, + "credited_solely_by_the_rule": 1236 + }, + "current": { + "records": 460, + "with_community_level": 391, + "mixed": 124, + "community_level_only": 267, + "taxa": 1507, + "credited_solely_by_the_rule": 1238 + } + }, + "interpretation": "KZ changes one pairwise interpretation to an explicitly two-member community-level observation; KMC narrows all three nodes to its four canonical core genera. Canonical taxonomy is unchanged. Census CL records390 to391, CL-only266 to267, taxa1505 to1507 and solely credited1236 to1238; no auditor-rule change." + }, + "network_warning_count": 36, + "corpus": { + "scope": "Every YAML under kb/communities and data/isolates; filesystem discovery includes hidden/ignored files.", + "records": 460, + "nodes": 1458, + "edges": 651, + "records_without_nodes": 8, + "records_without_edges": 156, + "review_status_counts": { + "needs_research": 21, + "pending": 245, + "reviewed": 194 + }, + "structural_defects": [] + }, + "tested_file_sha256": { + "reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml": "1e64150e4f7f3cde4b39dfc6bd97f2984ac3fcf26486b31bdbcdc710e2874a69", + "tests/test_causal_review_batch60.py": "dd1e198d098c1fb74896d4820253859100932c708930bd55c287af99174e2133", + "tests/test_participating_taxa.py": "27d2df501648e404a9d722844d513ebb29719034fc911348055d161842bf4a02", + "kb/communities/Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml": 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"83010c96524f844ac7ed56188e519e3878ee1f1ab33c2aa93f1a197920f78d6a", + "output_tail": "/Tropidoatractus_Magnetotacticus_Tripartite_Syntrophy.yaml\tCommunityMech:000337\tTropidoatractus magnetotacticus Magnetotactic Ciliate Tripartite Syntrophy\t3\t3\t0\t4\t2\t9\t\t0\t0\n450\t100.0\tkb/communities/Urine_Nitrification_SynCom.yaml\tCommunityMech:000117\tUrine Nitrification Synthetic Microbial Community\t5\t5\t0\t3\t2\t5\t\t0\t0\n451\t100.0\tkb/communities/Variovorax_Cryptococcus_Vitamin_Mutualism_Microcosm.yaml\tCommunityMech:000213\tVariovorax-Cryptococcus Vitamin Cross-Feeding Microcosm\t2\t2\t0\t3\t2\t4\t\t0\t0\n452\t100.0\tkb/communities/Waste_Sludge_Electrofermentation_Biofilm_Suspension_Community.yaml\tCommunityMech:000328\tWaste-Sludge Electro-Fermentation Biofilm-Suspension Community\t5\t5\t0\t4\t2\t6\t\t0\t0\n453\t100.0\tkb/communities/Watermelon_Rhizosphere_Fusarium_SynCom8.yaml\tCommunityMech:000098\tWatermelon Rhizosphere Fusarium-Protective SynCom8\t2\t2\t0\t3\t2\t7\t\t0\t0\n454\t100.0\tkb/communities/Wetland_Oxygen_Sulfate_GHG_Microcosm_Community.yaml\tCommunityMech:000255\tWetland Oxygen-Sulfate Greenhouse Gas Microcosm Community\t3\t3\t0\t3\t2\t6\t\t0\t0\n455\t100.0\tkb/communities/Wheat_Straw_Biogas_Pretreatment_SynCom.yaml\tCommunityMech:000118\tWheat Straw Biogas Pretreatment SynCom\t4\t4\t0\t3\t2\t4\t\t0\t0\n456\t100.0\tkb/communities/Yarrowia_lipolytica_Division_of_Labor_Lipid_Consortium.yaml\tCommunityMech:000354\tYarrowia lipolytica Division-of-Labor Lipid Consortium\t2\t2\t0\t3\t2\t3\t\t0\t0\n457\t100.0\tkb/communities/Yogurt_TwoSpecies_Starter_Culture.yaml\tCommunityMech:000164\tYogurt Two-Species Starter Culture\t2\t2\t0\t4\t2\t9\t\t0\t0\n458\t100.0\tkb/communities/Zymomonas_Ecoli_Exometabolomics_Obligate_Mutualism.yaml\tCommunityMech:000210\tZymomonas-E. coli Exometabolomics-Designed Obligate Mutualism\t2\t2\t0\t3\t2\t6\t\t0\t0\n459\t100.0\tkb/communities/hCom2_Complex_Gut_Microbiome.yaml\tCommunityMech:000123\thCom2 Complex Gut Microbiome\t1\t1\t0\t3\t2\t3\t\t0\t0\n460\t100.0\tkb/communities/mCAFEs_Brachypodium_RCC.yaml\tCommunityMech:000078\tm-CAFEs Brachypodium Reduced Complexity Consortia\t10\t10\t0\t6\t2\t11\t\t0\t0\nPYTHONPATH=src uv run python scripts/rank_causal_graph_readiness.py \n" + }, + { + "name": "just-network", + "command": [ + "just", + "audit-network-json" + ], + "exit_code": 2, + "expected_exit_code": 2, + "log": "/private/tmp/communitymech-batch60/baseline-just-network.log", + "sha256": "8595db43420d3f0a01c57acd6ad733a531a72e962486d20b4e1c2840dda55bff", + "output_tail": "uv run communitymech audit-network --json\nerror: Failed to spawn: `communitymech`\n Caused by: No such file or directory (os error 2)\nerror: recipe `audit-network-json` failed on line 574 with exit code 2\n" + }, + { + "name": "network", + "command": [ + "/Users/marcin/.cache/uv/archive-v0/l58VlyFvdmnkl0CcAx5Gk/bin/python3", + "-m", + "communitymech.cli", + "audit-network", + "--json" + ], + "exit_code": 1, + "expected_exit_code": 1, + "log": "/private/tmp/communitymech-batch60/baseline-network.log", + "sha256": "0955d3471f474fec8dcceb846ff932573d11c9833de73b2b2822241553762186", + "output_tail": " \"reference\": \"PMID:42577390\",\n \"supports\": \"SUPPORT\",\n \"evidence_source\": \"IN_VITRO\",\n \"snippet\": \"Staphylococcus capitis | K1-2-2-23 | ISS, V2A Steel | DSM 111179 | Sobisch et al., 2019 | GCA_025272695 | TSYB/TSYA | 37 \\u00b0C | 16\\u201324 h\",\n \"explanation\": \"Identifies S. capitis K1-2-2-23 as a consortium member and gives its source, DSM number, genome accession and cultivation conditions.\"\n }\n ]\n },\n \"message\": \"Taxon 'Staphylococcus capitis K1-2-2-23' has no interactions\"\n },\n {\n \"type\": \"DISCONNECTED\",\n \"taxon\": \"Streptococcus halotolerans HTS9T\",\n \"taxon_id\": \"NCBITaxon:1814128\",\n \"taxon_data\": {\n \"taxon_term\": {\n \"preferred_term\": \"Streptococcus halotolerans HTS9T\",\n \"term\": {\n \"id\": \"NCBITaxon:1814128\",\n \"label\": \"Streptococcus halotolerans\"\n },\n \"gtdb_grounding_status\": \"UNRESOLVED\",\n \"notes\": \"The source used the S. halotolerans type strain as a biosafety-level-1 representative.\"\n },\n \"strain_designation\": {\n \"strain_name\": \"HTS9T\",\n \"genome_accession\": \"GCA_001598035\",\n \"culture_collections\": [\n {\n \"collection\": \"DSM\",\n \"accession\": \"101996\"\n }\n ]\n },\n \"evidence\": [\n {\n \"reference\": \"PMID:42577390\",\n \"supports\": \"SUPPORT\",\n \"evidence_source\": \"IN_VITRO\",\n \"snippet\": \"Streptococcus halotolerans | HTS9 T | Respiratory Tract of Marmota himalayana | DSM 101996 T | Niu et al., 2016 | GCA_001598035 | 2xLB, TSYB/TSYA | 37 \\u00b0C | 24 h\",\n \"explanation\": \"Identifies S. halotolerans HTS9T as a consortium member and gives its source, DSM number, genome accession and cultivation conditions.\"\n }\n ]\n },\n \"message\": \"Taxon 'Streptococcus halotolerans HTS9T' has no interactions\"\n }\n ]\n}\n" + }, + { + "name": "downstream", + "command": [ + "rg", + "--no-ignore", + "--hidden", + "-n", + "downstream:", + "kb/communities", + "data/isolates", + "--glob", + "*.yaml" + ], + "exit_code": 0, + "expected_exit_code": 0, + "log": "/private/tmp/communitymech-batch60/baseline-downstream.log", + "sha256": "5832657aa07c09ba451e908d7b6e9ee1688df50ca7417fdd67605b8f273df21f", + "output_tail": "communities/Synechococcus_Saccharomyces_SPC.yaml:126: downstream:\nkb/communities/Synechococcus_Saccharomyces_SPC.yaml:161: downstream:\nkb/communities/PMI_Variovorax_Thermotolerance_Collection.yaml:330: downstream:\nkb/communities/Methylocystis_Rhodococcus_Methane_VFA_PHBV_Coculture.yaml:100: downstream:\nkb/communities/Methylocystis_Rhodococcus_Methane_VFA_PHBV_Coculture.yaml:144: downstream:\nkb/communities/Methylocystis_Rhodococcus_Methane_VFA_PHBV_Coculture.yaml:236: downstream:\nkb/communities/Methylotuvimicrobium_Synechococcus_Gas_Feedstock_Coculture.yaml:157: downstream:\nkb/communities/Methylotuvimicrobium_Synechococcus_Gas_Feedstock_Coculture.yaml:203: downstream:\nkb/communities/Trichoderma_Streptomyces_Filamentous_Cellulose_Coculture.yaml:135: downstream:\nkb/communities/Trichoderma_Streptomyces_Filamentous_Cellulose_Coculture.yaml:177: downstream:\nkb/communities/PET_Artificial_FourSpecies_Degradation_Consortium.yaml:190: downstream:\nkb/communities/PET_Artificial_FourSpecies_Degradation_Consortium.yaml:233: downstream:\nkb/communities/PET_Artificial_FourSpecies_Degradation_Consortium.yaml:273: downstream:\nkb/communities/PET_Artificial_FourSpecies_Degradation_Consortium.yaml:298: downstream:\nkb/communities/Rumen_Substrate_Adapted_Lignocellulose_Enrichment_Consortia.yaml:328: downstream:\nkb/communities/Synechococcus_Bacillus_SPC.yaml:120: downstream:\nkb/communities/Synechococcus_Bacillus_SPC.yaml:152: downstream:\nkb/communities/Mucin_Glycan_MDSC_Synthetic_Gut_Community.yaml:503: downstream:\nkb/communities/SynCom_Sesame_Flavor_Baijiu_Fuqu_13Genus.yaml:342: downstream:\nkb/communities/SynCom_Sesame_Flavor_Baijiu_Fuqu_13Genus.yaml:381: downstream:\nkb/communities/Engineered_Gut_Amino_Acid_CrossFeeding_Consortium.yaml:156: downstream:\nkb/communities/Engineered_Gut_Amino_Acid_CrossFeeding_Consortium.yaml:207: downstream:\nkb/communities/Pepper_Phytophthora_SynCom5.yaml:123: downstream:\nkb/communities/Pepper_Phytophthora_SynCom5.yaml:146: downstream:\n" + } + ], + "baseline_sha256": "ab4d9c40428b46f7bb6cc997595de77fbf97ec46189ca332940edecc90774bbf" + }, + "reference_validator_caveat": "CLI Total checks counts reported issues, not executed checks. Cache MATCH does not prove scientific entailment or complete provenance. All920 caches unchanged. KMC retained-arrow fragment independently matched to fresh publisher primary text. KZ correction body and full mechanism1541, empty Komagataella graph1542 and KMC non-graph1543 remain unresolved. Existing476/1091/1095 limitations remain." +} diff --git a/research/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-edison-literature-causal-meta.yaml b/research/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-edison-literature-causal-meta.yaml new file mode 100644 index 000000000..36710878b --- /dev/null +++ b/research/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture-edison-literature-causal-meta.yaml @@ -0,0 +1,128 @@ +slug: Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture +community_path: kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml +community_id: CommunityMech:000343 +job: LITERATURE +job_id: job-futurehouse-paperqa3 +label: causal +template_path: templates/community_causal_graph_research.md +template_vars: + community_name: Komagataella-E. coli Co-inducible Biosynthesis Coculture + community_id: CommunityMech:000343 + community_file: Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml + community_slug: Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture + community_category: BIOTECHNOLOGY + ecological_state: ENGINEERED + community_origin: SYNTHETIC + description: A defined two-member engineered yeast-bacterium consortium pairing Komagataella phaffii + with Escherichia coli for co-inducible relayed biosynthesis of natural products. Low-level glucose-responsive + and blue-light-responsive transcriptional systems were rebuilt separately for the two hosts so a single + signal could co-induce compound synthesis in coculture. After pathway redirection, genome mining, + and rewiring of acyl-donor degradation targets, the living consortium produced the reporter molecule + simvastatin from a simple carbon source and also supported inducible biosynthesis of (2S)-naringenin. + source_summary: '' + environment_summary: laboratory environment | ENVO:01001405 | The consortium was assayed under selected + co-growth conditions in a controlled coculture on a simple carbon source. + taxonomy_summary: Komagataella phaffii | Escherichia coli + interaction_summary: '' + environmental_factor_summary: Glucose and blue-light induction - low-level glucose- and blue light-responsive + transcriptional systems - Low-level glucose-responsive and blue-light-responsive transcriptional systems + were rebuilt separately for the host chassis so a single signal could co-induce compound synthesis + in the coculture. + growth_media_summary: Simple-carbon-source biosynthesis coculture - The abstract reports a living consortium + cultured on a simple carbon source but does not provide medium composition, induction schedule, incubation + temperature, or inoculation ratios. + dataset_summary: Primary publication - K. phaffii-E. coli co-inducible biosynthesis coculture - PMID:42492893 + - https://pubmed.ncbi.nlm.nih.gov/42492893/ + evidence_summary: 'PMID:42492893: This study explores yeast-bacterium consortium without engineered + interactions for the co-inducible relayed synthesis of natural products. (Supports the design goal + of a yeast-bacterium consortium for co-inducible relayed biosynthesis.) | PMID:42492893: Low-level + glucose- and blue light-responsive transcriptional systems are rebuilt separately for each host, allowing + single-signal co-induced activation of compound synthesis in coculture. (Supports the separately rebuilt + induction systems and single-signal coculture activation endpoint.) | PMID:42492893: The Komagataella + phaffii-Escherichia coli consortium is explored for co-growth under selected conditions. (Identifies + Komagataella phaffii as the yeast member of the two-species engineered consortium.) | PMID:42492893: + The Komagataella phaffii-Escherichia coli consortium is explored for co-growth under selected conditions. + (Identifies Escherichia coli as the bacterial member of the two-species engineered consortium.) | + PMID:42492893: Low-level glucose- and blue light-responsive transcriptional systems are rebuilt separately + for each host (Supports glucose and blue light response systems as engineered regulatory inputs in + the coculture design.) | PMID:42492893: cultured on simple carbon source (Supports the simple-carbon-source + cultivation condition used for the reporter simvastatin-producing living consortium.) | PMID:42492893: + Synthetic yeast-bacterium consortium enables co-inducible relayed synthesis of chemicals. (Lists the + exact primary publication for the K. phaffii-E. coli co-inducible biosynthesis coculture.)' +query_chars: 7457 +query: "# Microbial Community Causal-Graph Research Template\n\n## Target Community\n- **Community name:**\ + \ Komagataella-E. coli Co-inducible Biosynthesis Coculture\n- **Community id:** CommunityMech:000343\n\ + - **Community file:** Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml\n- **Community category:**\ + \ BIOTECHNOLOGY\n- **Ecological state:** ENGINEERED\n- **Community origin:** SYNTHETIC\n- **Environment:**\ + \ laboratory environment | ENVO:01001405 | The consortium was assayed under selected co-growth conditions\ + \ in a controlled coculture on a simple carbon source.\n- **Description:** A defined two-member engineered\ + \ yeast-bacterium consortium pairing Komagataella phaffii with Escherichia coli for co-inducible relayed\ + \ biosynthesis of natural products. Low-level glucose-responsive and blue-light-responsive transcriptional\ + \ systems were rebuilt separately for the two hosts so a single signal could co-induce compound synthesis\ + \ in coculture. After pathway redirection, genome mining, and rewiring of acyl-donor degradation targets,\ + \ the living consortium produced the reporter molecule simvastatin from a simple carbon source and also\ + \ supported inducible biosynthesis of (2S)-naringenin.\n\n## Existing Record Context\n- **Taxa:** Komagataella\ + \ phaffii | Escherichia coli\n- **Known ecological interactions:** \n- **Environmental factors:** Glucose\ + \ and blue-light induction - low-level glucose- and blue light-responsive transcriptional systems -\ + \ Low-level glucose-responsive and blue-light-responsive transcriptional systems were rebuilt separately\ + \ for the host chassis so a single signal could co-induce compound synthesis in the coculture.\n- **Growth\ + \ media / culture conditions:** Simple-carbon-source biosynthesis coculture - The abstract reports a\ + \ living consortium cultured on a simple carbon source but does not provide medium composition, induction\ + \ schedule, incubation temperature, or inoculation ratios.\n- **Associated datasets / resources:** Primary\ + \ publication - K. phaffii-E. coli co-inducible biosynthesis coculture - PMID:42492893 - https://pubmed.ncbi.nlm.nih.gov/42492893/\n\ + - **Existing evidence:** PMID:42492893: This study explores yeast-bacterium consortium without engineered\ + \ interactions for the co-inducible relayed synthesis of natural products. (Supports the design goal\ + \ of a yeast-bacterium consortium for co-inducible relayed biosynthesis.) | PMID:42492893: Low-level\ + \ glucose- and blue light-responsive transcriptional systems are rebuilt separately for each host, allowing\ + \ single-signal co-induced activation of compound synthesis in coculture. (Supports the separately rebuilt\ + \ induction systems and single-signal coculture activation endpoint.) | PMID:42492893: The Komagataella\ + \ phaffii-Escherichia coli consortium is explored for co-growth under selected conditions. (Identifies\ + \ Komagataella phaffii as the yeast member of the two-species engineered consortium.) | PMID:42492893:\ + \ The Komagataella phaffii-Escherichia coli consortium is explored for co-growth under selected conditions.\ + \ (Identifies Escherichia coli as the bacterial member of the two-species engineered consortium.) |\ + \ PMID:42492893: Low-level glucose- and blue light-responsive transcriptional systems are rebuilt separately\ + \ for each host (Supports glucose and blue light response systems as engineered regulatory inputs in\ + \ the coculture design.) | PMID:42492893: cultured on simple carbon source (Supports the simple-carbon-source\ + \ cultivation condition used for the reporter simvastatin-producing living consortium.) | PMID:42492893:\ + \ Synthetic yeast-bacterium consortium enables co-inducible relayed synthesis of chemicals. (Lists the\ + \ exact primary publication for the K. phaffii-E. coli co-inducible biosynthesis coculture.)\n\n## Research\ + \ Objective\n\nBuild a source-backed **causal interaction graph** for the microbial community\n**Komagataella-E.\ + \ coli Co-inducible Biosynthesis Coculture**, suitable for the `ecological_interactions` block of\n\ + `kb/communities/Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture.yaml`. The graph's **nodes are\ + \ mechanistic\ninteractions** and its **directed edges are causal links** (\"interaction A\nenables\ + \ / drives / suppresses interaction B\"). Extract only relationships the\nprimary literature supports;\ + \ do not infer edges that are not stated or directly\nimplied by an experiment.\n\n## Required Findings\n\ + \n### 1. Interaction nodes (the vertices)\n\nFor each mechanistic interaction, report as a table row:\n\ + \n- **name** — a short, unique interaction name (used as the node id and as the\n `target` of any causal\ + \ edge that points to it).\n- **interaction_type** — one of: MUTUALISM, COMMENSALISM, CROSS_FEEDING,\n\ + \ COMPETITION, PREDATION, SYNTROPHY, NICHE_PARTITIONING, STRAIN_COMPETITION,\n COLONIZATION_FACILITATION\ + \ (or state \"OTHER: \" if none fit).\n- **scope** — PAIRWISE (organism→organism) or COMMUNITY_LEVEL\ + \ (emergent /\n abiotically driven, e.g. electrolysis-supplied H2). PAIRWISE **must** name a\n source\ + \ taxon.\n- **source_taxon → target_taxon** — the directed pair (who acts on whom). Give\n NCBITaxon\ + \ CURIEs + labels where resolvable.\n- **metabolites / biological_processes** — the exchanged compound(s)\ + \ (CHEBI) and\n process(es) (GO) that mediate the interaction.\n- **direction / sign** — does the source\ + \ **increase (+)** or **decrease (−)** the\n target's growth/activity? State it explicitly.\n- **evidence**\ + \ — a short **verbatim** snippet + its reference (PMID/DOI).\n\n### 2. Causal edges (the arcs)\n\nThis\ + \ is the graph structure. For every ordered pair of nodes where the\nliterature supports a causal link,\ + \ report a row:\n\n- **from** — upstream interaction name (a node from §1).\n- **to** — downstream interaction\ + \ name (a node from §1).\n- **causal link** — one sentence: *how* the upstream interaction causes /\n\ + \ enables / is required for / suppresses the downstream one (e.g. \"H2 produced\n by the syntrophic\ + \ oxidation lowers pH2 enough for the acetogen to grow, which\n in turn supplies acetate to the methanogen\"\ + ).\n- **evidence** — verbatim snippet + reference for the causal claim specifically\n (not just for\ + \ the two endpoints existing).\n\nPrefer chains that a paper demonstrates by perturbation (knockout,\ + \ substrate\nremoval, inhibitor, co-culture vs mono-culture), and say which experiment shows\neach edge.\ + \ Flag any edge that is hypothesized rather than demonstrated.\n\n### 3. Grounding\n\n- CURIEs where\ + \ available: NCBITaxon (taxa), CHEBI (metabolites), GO (processes),\n ENVO (environment), PMID / DOI\ + \ (evidence).\n- Do not invent taxa, ontology ids, snippets, accession ids, or citations. If a\n node\ + \ or edge lacks a citable source, omit it or mark it UNVERIFIED.\n\n## Output Format\n\nReturn a curation-focused\ + \ report with:\n\n1. **Scope summary** — the consortium and the top-level function the causal graph\n\ + \ explains (1–3 sentences).\n2. **Node table** — the interaction nodes (§1 columns).\n3. **Edge table**\ + \ — the directed causal edges (§2 columns: from, to, causal\n link, evidence).\n4. **Adjacency / DOT\ + \ sketch** — a compact `from -> to` edge list (or Graphviz DOT\n snippet) so the graph can be eyeballed;\ + \ annotate edges with + / − sign.\n5. **Gaps & controversies** — edges the literature disputes, or plausible\ + \ edges\n with no direct evidence (candidates for a `Discussion`/knowledge-gap note).\n6. **References**\ + \ — every PMID/DOI cited, once.\n\nEach biological claim (node and edge) must carry a verbatim snippet\ + \ and a\nreference. This report is reviewed by a curator before anything is written to\n`kb/communities/`.\n" +submitted_at: '2026-10-06T20:12:05.069963+00:00' +status: dry-run +query_sha256: e429f0f613ac80c47e2e8ddfd83510abb8f87ba13c495f0d19a087207a5c33bb diff --git a/tests/test_causal_review_batch60.py b/tests/test_causal_review_batch60.py new file mode 100644 index 000000000..ab0648cc4 --- /dev/null +++ b/tests/test_causal_review_batch60.py @@ -0,0 +1,143 @@ +"""Pin KZ assay limits, KMC hypothesis scope and the unresolved empty relay graph.""" + +import hashlib +from pathlib import Path + +import yaml + +ROOT = Path(__file__).resolve().parents[1] +LEDGER = ROOT / "reports/causal_graph_review/decisions/20261006-kz-kmc-komagataella-batch60.yaml" +KZ = "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom" +KMC = "Kombucha_KMC_IMBG1_Fermentation_Community" +KOM = "Komagataella_Ecoli_Coinducible_Biosynthesis_Coculture" + + +def records(): + ledger = yaml.safe_load(LEDGER.read_text()) + rows = {Path(row["path"]).stem: row for row in ledger["records"]} + docs = {name: yaml.safe_load((ROOT / row["path"]).read_text()) for name, row in rows.items()} + return ledger, rows, docs + + +def test_kz_preserves_positive_assays_without_directed_transfer(): + _, rows, docs = records() + (node,) = docs[KZ]["ecological_interactions"] + assert rows[KZ]["status"] == "needs_research" + assert node["scope"] == "COMMUNITY_LEVEL" + assert not any( + key in node for key in ["source_taxon", "target_taxon", "interaction_type", "downstream"] + ) + assert [term["term"]["id"] for term in node["participating_taxa"]] == [ + "NCBITaxon:570", + "NCBITaxon:1663", + ] + assert "better phenanthrene degradation and mineralization" in node["description"] + assert "Biomass-normalized enzyme assays" in node["description"] + assert "not a resolved donor-to-recipient transfer" in node["description"] + assert node["evidence"][0]["supports"] == "PARTIAL" + assert node["evidence"][1]["supports"] == "SUPPORT" + + +def test_kz_correction_is_not_assumed_irrelevant_or_resolved(): + _, _, docs = records() + gaps = docs[KZ]["discussions"] + assert gaps[0]["discussion_id"] == "kz_cultivation_and_inoculation_details" + text = gaps[-1]["rationale"] + assert "PMID:41456976" in text + assert "metadata only, not its body" in text + assert "Do not assume the correction is biologically immaterial" in text + assert "#1541" in text and gaps[-1]["status"] == "OPEN" + + +def test_kmc_retains_the_source_explicit_spatial_model_as_hypothesized(): + ledger, rows, docs = records() + cooperation, pellicle, composition = docs[KMC]["ecological_interactions"] + assert len(rows[KMC]["edges_before"]) == 2 and len(rows[KMC]["edges_after"]) == 1 + assert cooperation["description"].startswith("HYPOTHESIZED - ") + assert cooperation["evidence"][0]["supports"] == "PARTIAL" + (edge,) = pellicle["downstream"] + assert edge["target"] == cooperation["name"] + assert edge["description"].startswith("HYPOTHESIZED - ") + assert "not a selective test" in edge["description"] + assert pellicle["evidence"][-1]["snippet"] == ledger["primary_matched_fragment"] + assert "downstream" not in composition + + +def test_kmc_preserves_composition_and_persistence_without_niche_or_mutualism_claim(): + _, _, docs = records() + cooperation, pellicle, composition = docs[KMC]["ecological_interactions"] + assert "not an exhaustive identical membership" in cooperation["description"] + assert "microscopy reports mixed bacterial and yeast morphotypes" in pellicle["description"] + assert "persisted through passages" in composition["description"] + assert ( + "relative sequence profiles do not establish absolute growth" in composition["description"] + ) + for node in [cooperation, pellicle, composition]: + assert "interaction_type" not in node and "source_taxon" not in node + assert [term["term"]["id"] for term in node["participating_taxa"]] == [ + "NCBITaxon:1434011", + "NCBITaxon:441", + "NCBITaxon:4919", + "NCBITaxon:13366", + ] + assert "#1543" in docs[KMC]["discussions"][-1]["rationale"] + + +def test_komagataella_empty_structure_remains_an_explicit_research_gap(): + ledger, rows, docs = records() + doc = docs[KOM] + assert "ecological_interactions" not in doc + assert rows[KOM]["status"] == "needs_research" + assert rows[KOM]["node_decisions"] == rows[KOM]["edges_after"] == [] + assert ledger["unresolved_research_issues"] == [1541, 1542] + assert doc["discussions"][0]["discussion_id"] == "kphaffii_ecoli_relayed_intermediate" + gap = doc["discussions"][-1] + assert gap["attaches_to"] == ["ecological_interactions", "engineering_design"] + assert "does not prove biological neutralism" in gap["rationale"] + assert "sharing an induction signal is not metabolite cross-feeding" in gap["rationale"] + assert "needs_research, not absent biology" in gap["rationale"] + + +def test_komagataella_edison_provenance_is_an_unpaid_single_community_dry_run(): + ledger, _, _ = records() + receipt = ledger["edison_dry_run"] + path = ROOT / receipt["path"] + assert hashlib.sha256(path.read_bytes()).hexdigest() == receipt["sha256"] + meta = yaml.safe_load(path.read_text()) + assert meta["community_id"] == "CommunityMech:000343" + assert meta["status"] == "dry-run" and meta["job"] == "LITERATURE" + assert len(meta["query"]) == meta["query_chars"] == 7457 + assert hashlib.sha256(meta["query"].encode()).hexdigest() == receipt["query_sha256"] + assert receipt["provider_submissions"] == receipt["credits_spent"] == 0 + + +def test_batch60_selected_cache_bytes_remain_unchanged(): + ledger, _, _ = records() + for path, digest in ledger["reference_cache_original_hashes"].items(): + assert hashlib.sha256((ROOT / path).read_bytes()).hexdigest() == digest + raw = (ROOT / "references_cache/PMID_26061774.md").read_text() + assert "".join(ledger["primary_matched_fragment"].split()) in "".join(raw.split()) + + +def test_batch60_every_node_arrow_and_new_anchor_is_accounted_for(): + ledger, rows, docs = records() + assert ledger["independent_approval"] is False + assert ledger["issues"] == [1539, 1540] + assert ledger["unresolved_non_graph_issues"] == [1543] + assert ledger["issue_deduplication"]["ignored_hidden_local_search"] is True + assert ( + len(rows[KMC]["retained_edge_decisions"]) == len(rows[KMC]["removed_edge_decisions"]) == 1 + ) + for name, row in rows.items(): + doc = docs[name] + assert hashlib.sha256((ROOT / row["path"]).read_bytes()).hexdigest() == row["record_sha256"] + assert doc["curation_history"][-1]["llm_assisted"] is True + assert len(row["history_files"]) == 1 + assert row["source_review"]["cache_provenance_certified"] is False + names = {node["name"] for node in doc.get("ecological_interactions", [])} + assert {decision["node"] for decision in row["node_decisions"]} == names + if name != KOM: + assert { + anchor.split("#", 1)[1] for anchor in doc["discussions"][-1]["attaches_to"] + } == names + assert doc["discussions"][-1]["status"] == "OPEN" diff --git a/tests/test_participating_taxa.py b/tests/test_participating_taxa.py index b368e2d52..162af691b 100644 --- a/tests/test_participating_taxa.py +++ b/tests/test_participating_taxa.py @@ -305,6 +305,8 @@ def test_a_curie_is_ambiguous_where_members_share_an_id(example): "KBase_ORT_Workflow_Community_Model.yaml", "KBase_Synthetic_Bacterial_Community_R2A.yaml", "Kefir_Flavor_Lentilactobacillus_Kluyveromyces_Coculture.yaml", + "Klebsiella_Arthrobacter_KZ_Phenanthrene_Cadmium_SynCom.yaml", + "Kombucha_KMC_IMBG1_Fermentation_Community.yaml", "Legume_Rhizobia_Mars_Simulant_Symbiosis.yaml", "Lunar_Simulant_Phosphate_Solubilizing_Bacteria_Nicotiana.yaml", "MSC1_Dominant_Core.yaml",