"increased representation of Pseudomonadales, Burkholderiales, and Sphingomonadales at the expense of Rhizobiales"
+
"The SynComs HR6 and HR8 were evaluated in pots filled with non-sterile soil from the UNLP experimental field at Los Hornos"
@@ -854,83 +725,51 @@
Evidence
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-
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HR6 Nonsterile-Soil Nodulation Promotion
- MUTUALISM
-
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The HR6+HN6 SynCom selected after six rounds promoted soybean nodule number, nodule dry weight, and shoot dry weight in non-sterile field soil compared with the uninoculated and B. japonicum E109 reference treatments.
-
+ KNOWLEDGE_GAP
+ OPEN
+ Which mechanisms explain the genotype and composition patterns, and what comparator effects are supported for HR6+HN6?
+
+
Three assembly/formulation arrows are removed; the previously pruned rhizosphere-to-HR6 arrow remains absent. The primary discussion leaves selection pressures unresolved, and co-occurrence/functional inference do not identify a mediator. For nodule number, total nodule dry weight and shoot dry weight, Table 1 shares significance letters between HR6+HN6 and both controls despite the narrative of higher values. Retain the reported means without blanket significance or field efficacy claims. Raw data and supplementary figures were not reanalyzed; candidate trait assays and non-graph roles are not recertified (#1821; related role queue #497).
+
Attaches to: ecological_interactions#Nodule-Associated Bradyrhizobium Genotype Pattern, ecological_interactions#Rhizosphere Community Composition Across Selection Rounds, ecological_interactions#HR6 Nonsterile-Soil Plant Phenotypes
unknown selection pressures seemed to shape the genotypic structure of the soybean-nodulating bradyrhizobial population at a rate faster than previously estimated.
+
The source leaves the selection mechanism unresolved.
+
-
"The SynComs HR6 and HR8 were evaluated in pots filled with non-sterile soil from the UNLP experimental field at Los Hornos"
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var width = container.clientWidth - 32;
// Dynamic height based on node count: minimum 500px, add 40px per node
- var nodeCount = 6;
+ var nodeCount = 5;
var height = Math.max(500, 400 + nodeCount * 20);
svg.attr("width", width).attr("height", height);
diff --git a/docs/communities/Soybean_N_Fixation_sfSynCom.html b/docs/communities/Soybean_N_Fixation_sfSynCom.html
index 0428108e4..e991e93eb 100644
--- a/docs/communities/Soybean_N_Fixation_sfSynCom.html
+++ b/docs/communities/Soybean_N_Fixation_sfSynCom.html
@@ -636,265 +636,68 @@
Taxonomy
Ecological Interactions
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-
The graph shows only explicitly linked interaction participants. Community members without an asserted participant link are omitted; membership alone does not imply an interaction.
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-
-
-
-
-
- Taxon
-
-
- Mutualism
-
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+
+
ℹ️ No detailed ecological interaction data is available for this community.
+
This record may be a metabolic model or minimal entry containing only taxonomy and external resource links.
+
-
-
-
AHL Quorum Sensing Signal Production
- MUTUALISM
-
-
Pantoea helper strain 1 produces acyl homoserine lactones that enhance Bradyrhizobium elkanii BXYD3 colonization and infection of soybean roots.
+ KNOWLEDGE_GAP
+ OPEN
+ What exact sfSynCom membership and source strain identities do the two ordinal Pantoea helper entries represent?
+
+
The abstract reports two Pantoea helper strains among the helpers and positive outcomes for the final sfSynCom, but does not establish that the final community contains only BXYD3 and those two helpers or map the ordinal labels to published strain identifiers. Three strain/formulation-attributed nodes and two arrows are withheld, not a rejection of the source's positive observations. Consortium-level improvement also does not establish reciprocal benefit for each encoded pair. EuropePMC supplied no PMCID; Wiley and journal-site full-text requests returned HTTP403. This is a bounded access and identity gap, not proof that full text or exact membership does not exist. Keep needs_research and resolve #1820 before restoring attribution. Identity/taxonomy and non-graph protocol blocks are preserved, not certified; no enhancement procedures are added.
-
- Downstream Effects:
-
- → Enhanced Root Colonization and Nodulation
-
Helper-derived AHL signaling feeds the enhanced Bradyrhizobium colonization, soybean infection, and nodulation phenotype.
"Two of these helper strains assigned to the Pantoea taxon produce acyl homoserine lactones, which significantly enhanced the colonization and infection of soybean by BXYD3"
-
-
-
-
-
-
-
-
-
-
AHL Quorum Sensing Signal Production (strain 2)
- MUTUALISM
-
-
Pantoea helper strain 2 also produces acyl homoserine lactones that support B. elkanii BXYD3 root colonization and infection in the sfSynCom.
Helper-derived AHL signaling feeds the enhanced Bradyrhizobium colonization, soybean infection, and nodulation phenotype.
+
Two of these helper strains assigned to the Pantoea taxon produce acyl homoserine lactones, which significantly enhanced the colonization and infection of soybean by BXYD3
+
Preserves the positive source observation but does not name the helper strains or establish the complete final formulation.
"Two of these helper strains assigned to the Pantoea taxon produce acyl homoserine lactones, which significantly enhanced the colonization and infection of soybean by BXYD3"
-
-
-
-
-
-
-
-
-
-
Enhanced Root Colonization and Nodulation
- MUTUALISM
-
-
Bradyrhizobium elkanii BXYD3 colonizes and infects soybean roots more effectively in the Pantoea-helper sfSynCom, improving nodulation and nitrogen fixation relative to BXYD3 alone.
This sfSynCom based on the core-helper strategy was more effective at promoting nodulation than inoculation with BXYD3 alone and achieved effects comparable to those of a complex elite SynCom previously constructed on the basis of potential beneficial functions between microbes and plants alone
+
Preserves the reported consortium-level comparison without assigning it to an unverified three-member formulation or reciprocal pairwise benefit.
+
-
"This sfSynCom based on the core-helper strategy was more effective at promoting nodulation than inoculation with BXYD3 alone and achieved effects comparable to those of a complex elite SynCom previously constructed on the basis of potential beneficial functions between microbes and plants alone"