Resazurin insoluble in stock solution (50mg/mL)

[kubu4]

The recipe for the resazurin stock solution uses a fairly high concentration of resazurin.

I've noticed that at this concentration, the resazurin seems to reach a saturation point, as a fair amount of the powder is insoluble, even with gentle heating/mixing.

What are other peoples' experiences?

Was the amount of resazurin needed for the stock solution ever "titered", or do these numbers stem from the original protocol provided to us?

In my opinion, we can be using far less resazurin in the stock solution, considering a significant portion is insoluble.

[AHuffmyer]

Yes, I have noticed this as well. It is based on recipes from all previous protocols and papers to ensure saturation. I noticed that solubility increased with gently warming, as you have tried. We could certainly try titrating to minimize any waste! Given how much we are using, minimizing would be more advantageous to us at this point.

[kubu4]

papers to ensure saturation.

Ah! Cool, thanks for confirming this!