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Bound carboxidivorans-kluyveri reactor mechanism and correct hexanoate grounding #1321

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Description

@realmarcin

Finding

Source-based self-adversarial review of CommunityMech:000266, not independent approval.

The two-stage node labels engineered reactor staging as NICHE_PARTITIONING and omits the pH change from 6 to 5 as well as covarying gas flow/residence time. The 800-mbar batch hexanol comparison is condition-specific, not a universal monoculture inability. The hexanoate metabolite is grounded to protonated hexanoic acid (CHEBI:30776), not hexanoate (CHEBI:17120).

Evidence And Acceptance

Read full main bodies, Methods, tables and captions of batch primary and cascade primary. Preserve supported reciprocal product cross-feeding and both existing arrows. Bound batch comparisons, describe combined reactor conditions without attributing the entire effect to CO alone, distinguish growth from ongoing chain elongation, and correct the C6 anion grounding using ChEBI. Add a focused mechanistic gap, append-only history, tests and regenerated page. No new nodes/arrows.

The cascade abstract says complete inhibition at 100 mbar, whereas Results report an 86% biomass reduction there and total inhibition at 800 mbar. Preserve that source discrepancy explicitly instead of importing the abstract number uncritically.

Duplicate check: 661 all-state issue titles/bodies plus ignored-inclusive local history/reports/research/tests; no duplicate within that scope. Comments not exhaustive.

Activity

  1. realmarcin commented on Oct 5, 2026

    @realmarcin
    ContributorAuthor

    Addressed in draft PR #1323 at db05129. Both conversion arrows remain; reactor pH/CO/flow/residence covariates, condition-specific batch comparison, de novo alcohol formation and the source growth-inhibition discrepancy are explicit. Hexanoate is corrected to official ChEBI:17120. Thirty local gates passed (243 regressions, 3 rights tests, 91 MATCH snippets); post-commit docs-current passed. Exact-head self-adversarial review: #1323 (review) . Keeping open until protected main merge; non-graph #1322 is separate.

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