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Bound cellobiose-biofilm causal graph claims to measured assays #1335

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@realmarcin

Source-based self-adversarial finding in Clostridium_Phytofermentans_Ecoli_Cellobiose_Biofilm_Consortium (CommunityMech:000196).

The graph combines planktonic substrate/ethanol measurements with biofilm biomass measurements, assigning all outputs to all three mechanisms. The primary Discussion expressly says biofilm cellobiose use and ethanol production were inferred, not directly measured. Polymer-cellulose process annotations also need separation from the tested cellobiose consortium.

The reciprocal necromass node has only a one-way pair; its peptide mediator is more certain than the assays. Oxygen partitioning is typed as colonization facilitation although the protective biofilm was established anoxically before oxygen exposure. The aggregate productivity endpoint is typed SYNTROPHY rather than distinguishing its constituent mechanisms.

Acceptance: preserve glucose-addition/cellobiase controls, both necromass-addition benefits, oxygen profiles, CFU evidence and condition-specific productivity. Account for all four nodes/three arrows, qualify mechanism contributions, distinguish qPCR from viability and biomass from EPS, and retain both canonical taxa. No new graph structure is required.

Primary: Park et al. 2020, PMID:33268782. Full main body, tables and captions read; images/supplements not inspected. Local ignored-inclusive search and 674 all-state issue titles/bodies checked; no duplicate exact graph issue in that scope. Broader enum #749/#752 remain separate. This is self-review, not independent approval.

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  1. realmarcin commented on Oct 6, 2026

    @realmarcin
    ContributorAuthor

    Addressed in #1339 at b4fd7d01144bf9815c3c5b5ce8c1c7b628a0a018. Exact-head source-based self-adversarial review: #1339 (review). All 30 local gates and actual post-commit docs-current passed; 262 passed in 220.78s (0:03:40); 83 MATCH snippets, zero other classes; 3 passed in 2.34s; 55 unrelated raw blocks and four caches byte-preserved. See committed batch21 decision/adversarial/validation records for assay bounds and full node/arrow accounting. This issue remains open until protected main merge; #1338 is separate non-graph work.

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