Fucoidan graph: bound combinatorial outcomes and curate complementary-enzyme causal structure #1465

CommunityMech:000449, Fucoidan_Seven_Degrader_Combinatorial_SynCom.yaml; PMID:42649294, doi:10.1038/s41586-026-10980-z.

The two existing nodes need explicit seven-degrader panel membership, experimental rather than solely computational guild support, and measurement limits. The 97.1% maximum belongs to the five-member F40/F56/V69/F94/V4 subset, not necessarily the complete seven-member mixture. Monomer depletion combines enzymatic cleavage and uptake, not CO2 mineralization. Functional synergism relative to the resource-partitioning null is not reciprocal fitness benefit; G88 is excluded from this panel and the mutualistic G88/V69 pair must not become a canonical interaction here. Initial OD was fixed per strain, so richness and total inoculum covary.

The full public primary body and Methods support complementary accessibility through cell-free residual substrate and purified-enzyme assays. This is not obligatory small-molecule cross-feeding or proof of each enzyme's necessity in every combination. New causal structure requires the one-community Edison workflow; dry run passed (15,343 characters), specific credit authorization requested, no provider job or spend. Keep needs_research until this expansion is actually assessed and curated. Meanwhile correct the existing nodes and add a focused gap without inventing an arrow.

Also correct the affected V4 discussion: the publisher DOM shows V4 followed by a superscript reference-3 link, not an alternative strain V43. Preserve the original selected-excerpt cache, G88 counter-selection, canonical taxonomy and unresolved GTDB mappings. Closed #1144-#1147 addressed different prior defects. No figure images, original supplementary spreadsheets, raw data or code were independently inspected; cached Table 2 selected rows were read.

Deduplication covered 783 all-state issue titles/bodies and ignored/hidden-inclusive local reports, research, history, tests, scripts and workflows, including exact PMIDs/DOIs. All 17 matched issue bodies read, not all comments or PR reviews. No exact duplicate found within that scope. This is source-based self-adversarial review, not independent approval.