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Geobacter-Methanosarcina graph: bound methane routes and hcp-mutant causal attribution #1481
CommunityMech:000033; Geobacter_Methanosarcina_DIET; PMID:24837373 and PMID:37650614.
The first node incorrectly describes Geobacter ethanol oxidation as acetate plus CO2, and the terminal node conflates net methane with DIET-only carbon reduction and turns a historical first report into timeless uniqueness. Preserve the DIET/pilin/GAC findings while bounding the terminal route and unknown methanogen entry components. For the 2023 hcp contrast, preserve the shorter establishment lag but remove NICHE_PARTITIONING and unmeasured secretion annotations. The discovery RNA-seq and GAC/AQDS comparisons use the G. sulfurreducens partner, not M. barkeri. EET transcript changes and faster Fe(III) oxide reduction expose pleiotropy; do not turn the negative model arrow into demonstrated T6SS antagonism or archaeal killing.
Acceptance: inspect all three existing nodes/two arrows and source/target/direction support; preserve the observed phenotype and explicitly hypothetical T6SS-specific attribution, with exact canonical participants and source-system boundaries. Add guarded history, focused tests, generated page and output records. New acetate-branch implementation is tracked separately; needs_research remains until that work is complete.
Source-based self-adversarial review, not independent approval. Whole records were read, with a pre-source both-root baseline from batch45 and a fresh unchanged-target/cache baseline for batch46. Complete primary main texts/Methods/captions were read for 2014 papers and the 2023 T6SS study. Selected 2014 figure images were inspected; no separate supplements, raw data or analysis code audited. All794 open/closed issue titles/bodies and ignored/hidden local review/history/research/tests/scripts/workflows searched; matched bodies #1091/#751/#306 read. Existing validator/cache issues are not duplicated. Not every issue comment or PR review inspected.
Existing-graph repair addressed; close only after protected main merge. #1484 at b59f9d6; exact-head self-adversarial review: #1484 (review). 461 passed in 211.31s (0:03:31); post-commit docs-current passed. Decisions, actual outputs and limitations committed. Not independent approval.
Activity
realmarcin commentedon Oct 6, 2026
Existing-graph repair addressed; close only after protected main merge. #1484 at b59f9d6; exact-head self-adversarial review: #1484 (review). 461 passed in 211.31s (0:03:31); post-commit docs-current passed. Decisions, actual outputs and limitations committed. Not independent approval.