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Separate added QS compounds from endogenous mutualism in the thermophilic pyrite graph #1893

Description

@realmarcin

Finding

CommunityMech:000073 presents single-species metabolic roles and externally dosed QS responses as pairwise mutualism/cross-feeding. The 2025 assays added DSF/BDSF at 2 uM each or four DL-AHLs at 5 uM each. Relative abundances and planktonic transcripts do not prove natural A. caldus AHL production, interpartner signal transfer, density-dependent resource protection, or uniform attachment responses. Retain the 2021 GC-MS evidence for Leptospirillum DSF production, distinguishing measured nM culture concentrations from uM additions and the niche-defense hypothesis. The 2020 dual culture had inoculation-order-dependent outcomes, not universal mutual benefit. Preserve the ferric/ferrous mineral loop; remove unsupported CBB attribution, transport-as-oxidation and overly specific compound mappings.

Sources And Limits

https://doi.org/10.3389/fmicb.2025.1592588
https://doi.org/10.1038/s41598-021-95324-9
https://doi.org/10.3389/fmicb.2020.00044

Whole canonical records and all existing graph snippets read. Fresh primary XML bodies for2025pyrite,2021DSF,2020dualculture,2021SCN read;2013eukaryote review read. The2003Tinto paper was retrieved as a13page institutional PDF after XML500 failures; main text and Figure6 reviewed. Two primary eukaryote/fungal abstracts traced from the review; no full-paper claim for these. Original pixels in other papers, supplements and raw data not audited. No new publication bodies or caches will be committed.

All-state title/body dedup includes closed issues697,694,543,513. Ignored/hidden-inclusive local path search excludes.git,docs,site,reference caches and.env; not an exhaustive historical PR-comment review.

Lifecycle

Address in batch115; close only after protected-main merge. Self-adversarial review is not independent approval.

Activity

  1. realmarcin commented on Oct 8, 2026

    @realmarcin
    ContributorAuthor

    Graph repair addressed; close only after protected-main merge. #1900 at 12eefd9; self-review #1900 (review). 1044 passed in 338.70s (0:05:38); postcommit docs-current passed. Three append-only histories, 34 validation outputs and seven mutation probes recorded. No new topology or Edison invocation in this batch; prior research extensions remain incomplete. Self-review is not independent approval.

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