perf(iVolcano): downsample tutorial data and link Hiplot full version

[ShixiangWang]

Summary

Resolves the A3.3 (iVolcano 页面瘦身) item of #306: the tutorial rendered 8 interactive widgets that each embedded the full example dataset as inline SVG (~46 MB per language, ~92 MB en+zh — the largest single page on the site).

Changes

Downsampling (data-preserving strategy)

• Omics/iVolcanoPlot.qmd + Omics/iVolcanoPlot.zh.qmd — in the load-data chunk, downsample df_limma before plotting: keep all significant genes (P.Value < 0.05 & |logFC| > 1, n = 1,950) plus a seed-fixed (set.seed(1)) random sample of 2,000 non-significant genes → 13,346 → 3,950 rows.
• Same treatment for df_airway in the pathway-linkage chunk: keep all genes with padj < 0.05 so GO enrichment inputs and the dot-plot ↔ volcano linkage are unchanged (20,606 → 5,755 rows).
• Comments in both languages explain the rationale and note that real analyses should use the full dataset.

Hiplot full-version link (外链完整版)

• Added callout-note blocks (en/zh) at the top of the page explaining the page uses downsampled data and linking the full-featured no-code Volcano app on Hiplot (openbiox's own platform), following the exact callout pattern used by Hiplot/183-volcano.qmd:
  • en: https://hiplot.cn/basic/volcano?lang=en
  • zh: https://hiplot.cn/basic/volcano?lang=zh_cn

Measured impact (local render, identical template)

Page	Before	After	Δ
iVolcanoPlot.html (one language)	48.2 MB	14.8 MB	−69%
en + zh deployed	~96 MB	~30 MB	−62 MB

(Widget blocks shrink 5.77–6.93 MB → 1.71–2.3 MB each; circle counts 13,346 → 3,950.)

Teaching value preserved (verified locally with set.seed(1))

• All 1,950 significant genes retained (Up 946 / Down 1,004 unchanged)
• All 10 extreme logFC > 8 genes retained → the filter = "logFC > 8" demo still labels all points
• Top-50 most significant gene extraction (fanyi chunk) still yields the same top genes
• enrichGO on the downsampled airway data returns the expected enriched terms; all 8 chunks execute cleanly end-to-end
• Interactive/static widget construction verified against installed ivolcano 0.0.5

Validation

• ✅ Full local render of the downsampled page (all 8 chunks, no errors)
• ✅ Downsampled vs full-data A/B render confirms only point counts shrink; widget structure/keys identical
• ✅ Both Hiplot links return HTTP 200
• ⏳ PR CI full render will validate the bilingual build

🤖 Generated with Claude Code

[github-actions]

✅ QMD Format Check

✅ All 1 checked QMD file(s) pass format validation!

Automated check by the PR Review workflow.
See contribution guidance for the expected tutorial format.

[github-actions]

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Modified files:

Omics/iVolcanoPlot.qmd Omics/iVolcanoPlot.zh.qmd