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Bound wheat treatment outcomes and inferred mechanisms (batch122) - #1953

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Scope

Closes #1947.
Closes #1948.
Closes #1949.
Closes #1950.
Non-graph source timing/figure and yield #1951-#1952 remain open; this batch does not resolve prior research extensions.
The genuine palette correction #1913 is inherited from the verified parent; required current-head CI remains a separate gate.

  • Preserve positive TB pot disease control without bilateral competition or individual-member necessity.; Keep relative community profiles and simulated robustness distinct from colonization facilitation and demonstrated immune mediation.
  • Retain positive barley protection without inferring inoculant superiority, hormone-pathway necessity or plant drought protection.; Preserve selective measured RNA changes and broad-profile nulls, with pre-challenge timing and no strain-level or causal-pathway assignment.
  • Retain enzyme-production output without reciprocal mutualism or attribution of downstream digestion to the inoculum.; Preserve polymer-degradation values and enzyme transfer rather than living-cell mutualism.; Keep positive downstream biogas yield distinct from methane-only yield or four-isolate methanogenesis.
  • Retain measured combined-treatment water retention while marking EPS mediation as hypothesized and coating-confounded.; Keep relative DNA-profile shifts distinct from unique-strain colonization, priority effects and predicted metabolic causality.

Adversarial Review

  • All nine nodes and both process directions retained,one rename,no new topology. No empty downstream lists,dangling targets or anchors. All four canonical diffs read after mutation; prior history entries and unrelated fields preserved.
  • TB retains positive70.97%pot efficacy,growth and grain-related outcomes without bilateral competition or unique immune mediation. Challenge fungus is not a TB member. No single-member plant arms demonstrate individual necessity. Simulated network attacks are not biological removal; genus assignments do not prove functions. Soil metabolites are not root-exudate collections; no compound-intervention evidence transferred from the different six-member study.
  • DT retains43.3%barley disease fluorescence reduction and no significant differences between inoculants. Specific JA/ET/SA dependence is not assigned. PEG-tolerant isolates are not whole-plant drought validation. Pre-challenge measured RNA changes retain the prior selective/not-broad evidence; contig similarity does not identify inoculant strain transcription.99%OTU matches do not establish unique viable strains.
  • Straw retains crude enzyme production,enzyme transfer and subsequent substrate digestion,both arrows and39.85/36.99/19.21%polymer degradation.801.16mL/gTS is positive total biogas,not methane-only yield. Removing the methane annotation does not claim methane absence. Four enzyme producers are not assumed to be the downstream methanogenic community; no reciprocal fitness inferred.
  • SASW retains combined BCC water and profile outcomes. EPS mediation remains HYPOTHESIZED with PARTIAL support; separate positive water evidence stays SUPPORT. BCC-versus-NC cannot isolate SynCom from coating. Relative profiles and ASV matching do not prove unique viable strains or priority effects; PICRUSt2 and correlations do not establish BCAA transfer or keystone necessity. Prior assembly/PICRUSt-node removals and uninspected-S1/S2 discussion preserved.
  • All17graph and6discussion quotes match fresh sources and unchanged caches. Three open main bodies read; straw abstract-bounded. Original figure pixels,external supplements,raw data and code not audited. No new cache content or protocol optimization. Source timing/figure and yield inconsistencies remain separate1951/1952.
  • Fifteen focused tests and12actual control/mutation/restored-control probes pass. Mutations reject restored competition/mutualism,erased TB efficacy,simulation-to-biological promotion,DT superiority and erased nulls,dropped straw arrow/degradation,methane annotation,erased coating confounding,EPS promotion and profile-to-colonization promotion. Canonical files unchanged by probes.
  • Four records pass schema,strict,terms,full-record references,snippets,rank and history.1161selected tests pass,not full Linux CI certification.57unchanged network warnings and zero structural defects. Exact descriptions on456pages,1359nodes,522arrows and deterministic rendering verified,not screenshot QA.
  • All456otherrecords,923caches and prior history/decision files unchanged. Participant lists and scopes unchanged:299list users,414community-level,136mixed,278community-only,1568taxa,1281solely credited. Scope credit is not actual-participant certification. No connectivity logic,threshold or schema changes.
  • Repairs1947-1950 addressed pending protected-main merge;1951/1952 remain non-graph follow-ups. Self-review is not independent approval. No Edison invocation or spend; Ruegeria1883 authentication blocker not retried unchanged. Root1836 and actual protected exact-head checks remain required.
  • Corpus396reviewed,10pending,54needs_research across460records. Overall objective,protected merge and branch deletion incomplete. Postcommit docs-current and exact-head publication are separate checks,not preasserted here.

Verification And Output Records

1161 passed in 600.56s (0:10:00); fifteen focused tests and twelve real in-memory mutation probes. All four records pass schema, strict, terms, full-record references, snippets, rank and history. 41 recorded gates retain commands, tails and digests. All 456 other records, 923 caches and unrelated raw fields are unchanged.

All nine nodes and both process arrows remain, one rename, no new topology. TB preserves positive disease control while distinguishing simulation and relative profiles from biological causality. DT preserves barley protection and selective pre-challenge RNA changes without pathway necessity or inoculant superiority. Straw keeps enzyme transfer, polymer degradation and total-biogas benefit without mutualism or methane-only attribution. SASW keeps combined biocoating outcomes with EPS mediation hypothesized and matrix confounding explicit.

All four whole records and abstracts reviewed; complete TB, DT and SASW open main bodies read. Straw is abstract-bounded. Original figure pixels, separate supplements, raw data and code not audited. All 23 graph/discussion quotations match fresh primary sources and unchanged caches; literal matching is not entailment proof.

Corpus: 396 reviewed, 10 pending, 54 needs_research. Network: 57 unchanged warnings, zero structural defects. Participant lists and scopes unchanged: 299 list users, 414 community-level, 136 mixed, 278 community-only, 1568 taxa, 1281 solely credited. No threshold, schema or connectivity changes. Exact descriptions on 456 pages, 1359 nodes and 522 arrows and deterministic rendering verified, not screenshot QA. Postcommit docs-current passed clean; log SHA256 3912f22ccd9a51831bb6170b49d4ab0ed9c93d51d901dac4773030175c04bd26. Selected local passes are not full Linux CI certification.

Decision ledger, provenance, validation, four append-only histories and self-adversarial review committed. Non-graph source timing/figure and yield #1951-#1952 remain open. No Edison invocation or spend; prior Ruegeria #1883 needs configured authentication. This COMMENT is self-review, not independent approval. Root #1836 requires actual protected checks. No artificial CI, protection bypass, merge or premature branch deletion.

Dependency

Draft depends on #1946. Do not merge into the topic parent. Require actual exact-head checks through protected main. Move dependents before deleting parent branches.

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Source-based self-adversarial review at exact head a8eb38f; not independent approval.

  • Preserve positive TB pot disease control without bilateral competition or individual-member necessity.; Keep relative community profiles and simulated robustness distinct from colonization facilitation and demonstrated immune mediation.

  • Retain positive barley protection without inferring inoculant superiority, hormone-pathway necessity or plant drought protection.; Preserve selective measured RNA changes and broad-profile nulls, with pre-challenge timing and no strain-level or causal-pathway assignment.

  • Retain enzyme-production output without reciprocal mutualism or attribution of downstream digestion to the inoculum.; Preserve polymer-degradation values and enzyme transfer rather than living-cell mutualism.; Keep positive downstream biogas yield distinct from methane-only yield or four-isolate methanogenesis.

  • Retain measured combined-treatment water retention while marking EPS mediation as hypothesized and coating-confounded.; Keep relative DNA-profile shifts distinct from unique-strain colonization, priority effects and predicted metabolic causality.

  • All nine nodes and both process directions retained,one rename,no new topology. No empty downstream lists,dangling targets or anchors. All four canonical diffs read after mutation; prior history entries and unrelated fields preserved.

  • TB retains positive70.97%pot efficacy,growth and grain-related outcomes without bilateral competition or unique immune mediation. Challenge fungus is not a TB member. No single-member plant arms demonstrate individual necessity. Simulated network attacks are not biological removal; genus assignments do not prove functions. Soil metabolites are not root-exudate collections; no compound-intervention evidence transferred from the different six-member study.

  • DT retains43.3%barley disease fluorescence reduction and no significant differences between inoculants. Specific JA/ET/SA dependence is not assigned. PEG-tolerant isolates are not whole-plant drought validation. Pre-challenge measured RNA changes retain the prior selective/not-broad evidence; contig similarity does not identify inoculant strain transcription.99%OTU matches do not establish unique viable strains.

  • Straw retains crude enzyme production,enzyme transfer and subsequent substrate digestion,both arrows and39.85/36.99/19.21%polymer degradation.801.16mL/gTS is positive total biogas,not methane-only yield. Removing the methane annotation does not claim methane absence. Four enzyme producers are not assumed to be the downstream methanogenic community; no reciprocal fitness inferred.

  • SASW retains combined BCC water and profile outcomes. EPS mediation remains HYPOTHESIZED with PARTIAL support; separate positive water evidence stays SUPPORT. BCC-versus-NC cannot isolate SynCom from coating. Relative profiles and ASV matching do not prove unique viable strains or priority effects; PICRUSt2 and correlations do not establish BCAA transfer or keystone necessity. Prior assembly/PICRUSt-node removals and uninspected-S1/S2 discussion preserved.

  • All17graph and6discussion quotes match fresh sources and unchanged caches. Three open main bodies read; straw abstract-bounded. Original figure pixels,external supplements,raw data and code not audited. No new cache content or protocol optimization. Source timing/figure and yield inconsistencies remain separate1951/1952.

  • Fifteen focused tests and12actual control/mutation/restored-control probes pass. Mutations reject restored competition/mutualism,erased TB efficacy,simulation-to-biological promotion,DT superiority and erased nulls,dropped straw arrow/degradation,methane annotation,erased coating confounding,EPS promotion and profile-to-colonization promotion. Canonical files unchanged by probes.

  • Four records pass schema,strict,terms,full-record references,snippets,rank and history.1161selected tests pass,not full Linux CI certification.57unchanged network warnings and zero structural defects. Exact descriptions on456pages,1359nodes,522arrows and deterministic rendering verified,not screenshot QA.

  • All456otherrecords,923caches and prior history/decision files unchanged. Participant lists and scopes unchanged:299list users,414community-level,136mixed,278community-only,1568taxa,1281solely credited. Scope credit is not actual-participant certification. No connectivity logic,threshold or schema changes.

  • Repairs1947-1950 addressed pending protected-main merge;1951/1952 remain non-graph follow-ups. Self-review is not independent approval. No Edison invocation or spend; Ruegeria1883 authentication blocker not retried unchanged. Root1836 and actual protected exact-head checks remain required.

  • Corpus396reviewed,10pending,54needs_research across460records. Overall objective,protected merge and branch deletion incomplete. Postcommit docs-current and exact-head publication are separate checks,not preasserted here.

1161 passed in 600.56s (0:10:00); fifteen focused tests and twelve real in-memory mutation probes. All four records pass schema, strict, terms, full-record references, snippets, rank and history. 41 recorded gates retain commands, tails and digests. All 456 other records, 923 caches and unrelated raw fields are unchanged.

All nine nodes and both process arrows remain, one rename, no new topology. TB preserves positive disease control while distinguishing simulation and relative profiles from biological causality. DT preserves barley protection and selective pre-challenge RNA changes without pathway necessity or inoculant superiority. Straw keeps enzyme transfer, polymer degradation and total-biogas benefit without mutualism or methane-only attribution. SASW keeps combined biocoating outcomes with EPS mediation hypothesized and matrix confounding explicit.

All four whole records and abstracts reviewed; complete TB, DT and SASW open main bodies read. Straw is abstract-bounded. Original figure pixels, separate supplements, raw data and code not audited. All 23 graph/discussion quotations match fresh primary sources and unchanged caches; literal matching is not entailment proof.

Corpus: 396 reviewed, 10 pending, 54 needs_research. Network: 57 unchanged warnings, zero structural defects. Participant lists and scopes unchanged: 299 list users, 414 community-level, 136 mixed, 278 community-only, 1568 taxa, 1281 solely credited. No threshold, schema or connectivity changes. Exact descriptions on 456 pages, 1359 nodes and 522 arrows and deterministic rendering verified, not screenshot QA. Postcommit docs-current passed clean; log SHA256 3912f22ccd9a51831bb6170b49d4ab0ed9c93d51d901dac4773030175c04bd26. Selected local passes are not full Linux CI certification.

Decision ledger, provenance, validation, four append-only histories and self-adversarial review committed. Non-graph source timing/figure and yield #1951-#1952 remain open. No Edison invocation or spend; prior Ruegeria #1883 needs configured authentication. This COMMENT is self-review, not independent approval. Root #1836 requires actual protected checks. No artificial CI, protection bypass, merge or premature branch deletion.

@github-actions

github-actions Bot commented Oct 8, 2026

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Network integrity findings

Warnings only — a member with no interaction yet, or a participant matched by ontology id rather than by name, or one on a community-level interaction that resolves to no member. Reported, but does not fail the build.

Network Integrity Audit Report
================================================================================

0 error, 57 warning across 21 records with findings
Only error-severity findings fail the build.

Algal_Methanotroph_Biogas_Valorization_Coculture
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Leptolyngbya' has no interactions

Total: 1 issues (0 error, 1 warning)

At_RSPHERE_SynCom
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Flavobacterium sp.' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonas sp.' has no interactions
  [warning] DISCONNECTED: Taxon 'Rhodococcus sp.' has no interactions
  [warning] DISCONNECTED: Taxon 'Streptomyces sp.' has no interactions
  [warning] DISCONNECTED: Taxon 'Variovorax sp.' has no interactions

Total: 5 issues (0 error, 5 warning)

Australian_Lead_Zinc_Polymetallic
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Acidithiobacillus ferrooxidans' has no interactions
  [warning] DISCONNECTED: Taxon 'Leptospirillum ferriphilum' has no interactions

Total: 2 issues (0 error, 2 warning)

Bacillus_G12_Y4_X25_Tobacco_Biocontrol_SynCom
--------------------------------------------------------------------------------
  [warning] UNKNOWN_TARGET: Target taxon 'tobacco' not found in taxonomy section (community-level scope)
  [warning] UNKNOWN_TARGET: Target taxon 'tobacco' not found in taxonomy section (community-level scope)

Total: 2 issues (0 error, 2 warning)

Bacillus_siamensis_vallismortis_HT_Masson_Pine_SynCom
--------------------------------------------------------------------------------
  [warning] UNKNOWN_TARGET: Target taxon 'Fusarium oxysporum' not found in taxonomy section (community-level scope)
  [warning] UNKNOWN_TARGET: Target taxon 'Pinus massoniana' not found in taxonomy section (community-level scope)
  [warning] UNKNOWN_TARGET: Target taxon 'Pinus massoniana' not found in taxonomy section (community-level scope)

Total: 3 issues (0 error, 3 warning)

Brocadia_Low_Nitrogen_Anammox_Consortium
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Ca. Kuenenia early-acclimation lineage' has no interactions

Total: 1 issues (0 error, 1 warning)

Dangl_SynComm_35
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Escherichia coli DH5α' has no interactions
  [warning] DISCONNECTED: Taxon 'Flavobacterium species' has no interactions
  [warning] DISCONNECTED: Taxon 'Microbacterium species' has no interactions
  [warning] DISCONNECTED: Taxon 'Stenotrophomonas species' has no interactions
  [warning] DISCONNECTED: Taxon 'Xanthomonadales members' has no interactions

Total: 5 issues (0 error, 5 warning)

Deepwater_Horizon_Deep_Sea_Oil_Plume_Succession
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Methylophaga' has no interactions

Total: 1 issues (0 error, 1 warning)

East_River_Floodplain_Core_Microbiome
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Acidobacteriota floodplain representatives' has no interactions
  [warning] DISCONNECTED: Taxon 'Nitrospirae core floodplain members' has no interactions

Total: 2 issues (0 error, 2 warning)

GLBRC_UFMP_Fermentation_Community
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'RUG023 sp. (MAG SPH2)' has no interactions

Total: 1 issues (0 error, 1 warning)

KBase_Models_for_Zahmeeth_Original_PLOS
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Klebsiella' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonas fluorescens' has no interactions

Total: 2 issues (0 error, 2 warning)

Lake_Washington_Methane_Oxygen_Methylotroph_Community
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Methylosarcina' has no interactions

Total: 1 issues (0 error, 1 warning)

Lotus_LjSC3
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Burkholderiaceae bacterium' has no interactions
  [warning] DISCONNECTED: Taxon 'Mesorhizobium sp. LjNodule210' has no interactions
  [warning] DISCONNECTED: Taxon 'Mesorhizobium sp. LjNodule215' has no interactions
  [warning] DISCONNECTED: Taxon 'Mesorhizobium sp. LjNodule218' has no interactions
  [warning] DISCONNECTED: Taxon 'Microbacteriaceae bacterium' has no interactions
  [warning] DISCONNECTED: Taxon 'Oxalobacteriaceae bacterium' has no interactions
  [warning] DISCONNECTED: Taxon 'Phyllobacteriaceae bacterium' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonadaceae bacterium' has no interactions
  [warning] DISCONNECTED: Taxon 'Rhizobiaceae bacterium' has no interactions

Total: 9 issues (0 error, 9 warning)

Nitrifying_Wastewater_Ammonia_Oxidizing_Consortium
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Legionella MAGs' has no interactions

Total: 1 issues (0 error, 1 warning)

Panzhihua_Vanadium_Titanium_Tailings
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Polaromonas species' has no interactions
  [warning] DISCONNECTED: Taxon 'Rhizobium pisi' has no interactions
  [warning] DISCONNECTED: Taxon 'Rhizobium selenitireducens' has no interactions

Total: 3 issues (0 error, 3 warning)

Phormidium_Alkaline_Consortium
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Bacteroidota members' has no interactions

Total: 1 issues (0 error, 1 warning)

Polaromonas_Vanadium_Reduction_Community
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Desulfitobacterium species' has no interactions
  [warning] DISCONNECTED: Taxon 'Desulfovibrio species' has no interactions
  [warning] DISCONNECTED: Taxon 'Geobacter species' has no interactions

Total: 3 issues (0 error, 3 warning)

Pseudomonas_Aeruginosa_EightStrain_Full_Factorial_SynCom
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Pseudomonas aeruginosa MDR6-CAZ' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonas aeruginosa NfxB-CAZ' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonas aeruginosa OrfN' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonas aeruginosa PA14' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonas aeruginosa mexZ' has no interactions

Total: 5 issues (0 error, 5 warning)

Sclerotinia_Sclerotia_12Strain_Biocontrol_SynCom
--------------------------------------------------------------------------------
  [warning] UNKNOWN_TARGET: Target taxon 'Sclerotinia sclerotiorum' not found in taxonomy section (community-level scope)
  [warning] UNKNOWN_TARGET: Target taxon 'Sclerotinia sclerotiorum' not found in taxonomy section (community-level scope)

Total: 2 issues (0 error, 2 warning)

Space_Habitat_SevenMember_Stress_Tolerance_SynCom
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Acinetobacter radioresistens 50V1' has no interactions
  [warning] DISCONNECTED: Taxon 'Micrococcus luteus W4-Al-K1' has no interactions
  [warning] DISCONNECTED: Taxon 'Propionibacterium cyclohexanicum TA-12T' has no interactions
  [warning] DISCONNECTED: Taxon 'Pseudomonas antarctica CMS 35T' has no interactions
  [warning] DISCONNECTED: Taxon 'Staphylococcus capitis K1-2-2-23' has no interactions
  [warning] DISCONNECTED: Taxon 'Streptococcus halotolerans HTS9T' has no interactions

Total: 6 issues (0 error, 6 warning)

Tinto_River_Iron_Cycling_Community
--------------------------------------------------------------------------------
  [warning] DISCONNECTED: Taxon 'Basidiomycota' has no interactions

Total: 1 issues (0 error, 1 warning)

The full report is attached to the workflow run as an artifact.

@realmarcin
realmarcin added this pull request to stack #1973 October 8, 2026 18:56
@realmarcin
realmarcin force-pushed the causal-graph-review-20261005-batch121 branch from ec8ee08 to d018b9a Compare October 8, 2026 19:04
@realmarcin
realmarcin force-pushed the causal-graph-review-20261005-batch122 branch from a8eb38f to 0e12db5 Compare October 8, 2026 19:04

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Exact-head self-adversarial restack review at 0e12db50706409d42cb89436f3d385f08cdc6a1f.
COMMENT, not independent approval.

The batch-owned binary patch remains byte-identical:
SHA-256 487273517a45efb6043fc042b4d36f53f718ab87411444b5c17aaefb39bebb19.
Tree eaa34b21448f1df3873d7ae4f129d84d5c64e504 matches the independently computed clean merge.
Compared with historical tree 8b26ed5f8364da0c76565678d7cf40111db9ab43, only scripts/.vendored_canon_ref
changes, incorporating main PR #1834's pin 849f336e025510316a5f235eb0af8547b8bd50cc.
All curated records, source caches, review outputs, histories, regression tests, and rendered
pages remain unchanged. No new primary-source review or local scientific-test rerun is claimed.

This PR is now part of native stack #1973, rooted at #1363 on main. No protected merge
has occurred. Fresh eligible PR/merge-group CI remains mandatory. No rule bypass, fabricated
status, merge into a topic parent, or premature branch deletion.

@realmarcin
realmarcin marked this pull request as ready for review October 8, 2026 19:13
@realmarcin
realmarcin force-pushed the causal-graph-review-20261005-batch122 branch from 0e12db5 to cdcbdec Compare October 9, 2026 08:02
@realmarcin
realmarcin removed this pull request from stack #1973 October 10, 2026 02:32
@realmarcin

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Superseded by the protected cumulative merge #1975 at 42959bef2549f9a42c3e3303a0e10fd5be99f30e. This PR's reviewed source head cdcbdecdb63092a0a9d46ef2f317e8645c5052b3 was included in the cumulative branch; the final squash tree matches the reviewed integration tree exactly, and all seven required merge-group checks passed.

Patch-inclusion receipt. Existing issue-closing links were transferred to #1975; unresolved research and non-graph gaps are not closed by this action. This PR is being closed as superseded, not represented as individually merged. Archival provenance tags retain source commits; cleanup removes branch refs only and preserves worktree files.

@realmarcin realmarcin closed this Oct 10, 2026
@realmarcin
realmarcin deleted the causal-graph-review-20261005-batch122 branch October 10, 2026 02:42
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